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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1092 [new locus tag: SACOL_RS05575 ]
  • pan locus tag?: SAUPAN003304000
  • symbol: ptsI
  • pan gene symbol?: ptsI
  • synonym:
  • product: phosphoenolpyruvate-protein phosphotransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1092 [new locus tag: SACOL_RS05575 ]
  • symbol: ptsI
  • product: phosphoenolpyruvate-protein phosphotransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1102054..1103772
  • length: 1719
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCTAAATTAATTAAAGGTATTGCCGCATCTGATGGTGTCGCAATTGCTAAAGCTTAT
    TTATTAGTTGAGCCAGACTTAACATTCGACAAAAATGAAAAAGTCACTGATGTTGAAGGA
    GAAGTTGCAAAGTTCAATAGCGCTATCGAAGCTTCTAAAGTTGAGTTAACTAAAATTAGA
    AATAATGCAGAGGTTCAACTAGGTGCTGATAAAGCTGCTATCTTTGATGCACATTTATTA
    GTTTTAGATGACCCTGAATTAATTCAACCAATCCAAGATAAGATTAAAAATGAAAACGCT
    AATGCTGCTACAGCATTAACGGATGTAACAACACAATTTGTTACAATTTTTGAATCTATG
    GATAACGAATACATGAAAGAACGTGCGGCTGATATTCGCGACGTTTCTAAACGTGTGTTA
    TCACATATTTTAGGTGTAGAATTACCGAATCCGAGTATGATTGATGAAAGCGTTGTTATT
    GTAGGGAATGACTTAACGCCATCTGATACTGCTCAATTAAATAAAGAATTCGTACAAGGT
    TTTGCTACAAACATTGGCGGAAGAACAAGTCACTCTGCAATTATGAGTCGTTCTTTAGAA
    ATTCCAGCAATTGTTGGTACAAAATCAATTACTCAAGAAGTTAAACAAGGCGACATGATT
    ATCGTAGATGGATTAAATGGTGATGTAATCGTTAATCCAACTGAAGATGAGTTAATCGCT
    TATCAAGATAAACGTGAGCGTTATTTTGCTGACAAGAAAGAATTACAAAAACTACGTGAT
    GCTGATACTGTTACAGTTGATGGTGTTCACGCAGAGCTTGCTGCAAATATTGGTACACCT
    AATGATTTGCCAGGTGTTATTGAAAATGGTGCACAAGGTATCGGCTTATATAGAACTGAG
    TTTTTATATATGGGTCGTGACCAAATGCCTACAGAAGAAGAACAATTTGAAGCTTATAAA
    GAAGTATTAGAAGCAATGGGCGGTAAACGTGTTGTTGTACGTACTTTAGATATAGGTGGA
    GATAAAGAATTATCATACTTAAACTTGCCTGAAGAAATGAATCCATTCTTAGGTTACCGT
    GCGATTCGTTTATGCCTTGCGCAACAAGATATTTTCAGACCACAGCTACGTGCATTATTA
    CGTGCATCAGTTTATGGTAAGTTAAATATCATGTTCCCAATGGTTGCAACAATTAACGAA
    TTTAGAGAAGCTAAAGCTATATTATTAGAAGAAAAAGAAAACCTTAAAAATGAAGGTCAT
    GACATTTCGGATGATATAGAATTAGGAATCATGGTAGAGATACCTGCAACAGCAGCATTA
    GCTGATGTCTTTGCTAAAGAAGTAGATTTCTTCAGTATCGGTACAAATGATTTAATTCAA
    TACACATTAGCTGCTGACCGTATGTCAGAGCGTGTATCATATCTATACCAACCATATAAC
    CCTTCAATCTTACGTTTAGTTAAACAAGTTATTGAAGCGTCACATAAAGAAGGTAAATGG
    ACAGGTATGTGTGGTGAAATGGCTGGAGATGAAACAGCTATTCCATTATTGCTTGGTTTA
    GGTTTAGATGAGTTCTCTATGAGTGCAACGTCTATTCTGAAAGCAAGAAGACAAATTAAT
    GGTTTAAGTAAAAATGAAATGACTGAACTTGCTAACCGTGCAGTCGACTGTGCAACGCAA
    GAAGAAGTTATTGAATTAGTTAACAACTACGTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1719

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1092 [new locus tag: SACOL_RS05575 ]
  • symbol: PtsI
  • description: phosphoenolpyruvate-protein phosphotransferase
  • length: 572
  • theoretical pI: 4.38638
  • theoretical MW: 63218.6
  • GRAVY: -0.174126

Function[edit source | edit]

  • reaction:
    EC 2.7.3.9?  ExPASy
    Phosphoenolpyruvate--protein phosphotransferasePhosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine
  • TIGRFAM:
    phosphoenolpyruvate-protein phosphotransferase (TIGR01417; EC 2.7.3.9; HMM-score: 816.2)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisphosphoenolpyruvate synthase (TIGR01418; EC 2.7.9.2; HMM-score: 209.3)
    MetabolismEnergy metabolismOtherpyruvate, phosphate dikinase (TIGR01828; EC 2.7.9.1; HMM-score: 135.6)
  • TheSEED:  
    CarbohydratesMonosaccharidesFructose utilization Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9) 
    Sugar alcoholsMannitol Utilization Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9) 
  • PFAM:
    PK_TIM (CL0151) PEP-utilizers_C; PEP-utilising enzyme, TIM barrel domain (PF02896; HMM-score: 426.5)
    no clan definedPEP-utilisers_N; PEP-utilising enzyme, N-terminal (PF05524; HMM-score: 111.9)
    Leu-IlvD (CL0364) PEP-utilizers; PEP-utilising enzyme, mobile domain (PF00391; HMM-score: 83.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:
    SACOL1760(ackA)acetate kinase  [1] (data from MRSA252)
    SACOL2657(arcA)arginine deiminase  [1] (data from MRSA252)
    SACOL0002(dnaN)DNA polymerase III subunit beta  [1] (data from MRSA252)
    SACOL1199(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    SACOL2415(gpmA)phosphoglyceromutase  [1] (data from MRSA252)
    SACOL1741(icd)isocitrate dehydrogenase  [1] (data from MRSA252)
    SACOL1288(infB)translation initiation factor IF-2  [1] (data from MRSA252)
    SACOL1102(pdhA)pyruvate dehydrogenase complex E1 component subunit alpha  [1] (data from MRSA252)
    SACOL1103(pdhB)pyruvate dehydrogenase complex E1 component subunit beta  [1] (data from MRSA252)
    SACOL1104(pdhC)branched-chain alpha-keto acid dehydrogenase E2  [1] (data from MRSA252)
    SACOL1105(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SACOL2128(pdp)pyrimidine-nucleoside phosphorylase  [1] (data from MRSA252)
    SACOL0204(pflB)formate acetyltransferase  [1] (data from MRSA252)
    SACOL1745(pyk)pyruvate kinase  [1] (data from MRSA252)
    SACOL0584(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SACOL2236(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    SACOL2239(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SACOL2238(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    SACOL2224(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    SACOL0585(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SACOL0583(rplK)50S ribosomal protein L11  [1] (data from MRSA252)
    SACOL0586(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    SACOL2207(rplM)50S ribosomal protein L13  [1] (data from MRSA252)
    SACOL2220(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SACOL2223(rplR)50S ribosomal protein L18  [1] (data from MRSA252)
    SACOL1257(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SACOL1725(rplT)50S ribosomal protein L20  [1] (data from MRSA252)
    SACOL1702(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SACOL2234(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    SACOL2237(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    SACOL1238(rpmB)50S ribosomal protein L28  [1] (data from MRSA252)
    SACOL2112(rpmE2)50S ribosomal protein L31  [1] (data from MRSA252)
    SACOL2213(rpoA)DNA-directed RNA polymerase subunit alpha  [1] (data from MRSA252)
    SACOL2233(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SACOL2222(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SACOL0592(rpsG)30S ribosomal protein S7  [1] (data from MRSA252)
    SACOL2225(rpsH)30S ribosomal protein S8  [1] (data from MRSA252)
    SACOL2206(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    SACOL2214(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    SACOL2215(rpsM)30S ribosomal protein S13  [1] (data from MRSA252)
    SACOL1254(rpsP)30S ribosomal protein S16  [1] (data from MRSA252)
    SACOL2230(rpsQ)30S ribosomal protein S17  [1] (data from MRSA252)
    SACOL0439(rpsR)30S ribosomal protein S18  [1] (data from MRSA252)
    SACOL2235(rpsS)30S ribosomal protein S19  [1] (data from MRSA252)
    SACOL1722(tig)trigger factor  [1] (data from MRSA252)
    SACOL0594(tuf)elongation factor Tu  [1] (data from MRSA252)
    SACOL2104(upp)uracil phosphoribosyltransferase  [1] (data from MRSA252)
    SACOL0688ABC transporter substrate-binding protein  [1] (data from MRSA252)
    SACOL2173alkaline shock protein 23  [1] (data from MRSA252)
    SACOL25691-pyrroline-5-carboxylate dehydrogenase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.18
    • Ymax_pos: 18
    • Cmax: 0.122
    • Cmax_pos: 18
    • Smax: 0.39
    • Smax_pos: 17
    • Smean: 0.253
    • D: 0.208
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAKFNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANAATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVIVGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGDMIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAANIGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMGGKRVVVRTLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMFPMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDFFSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMAGDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIELVNNYVK

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [2] [3] [4] [5]
    quantitative data / protein copy number per cell: 4926 [6]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: 57.03 h [7]

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  3. Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J. Proteome Res.: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  4. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J. Proteome Res.: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  5. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  6. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  7. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol. Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]