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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1885 [new locus tag: SA_RS10845 ]
  • pan locus tag?: SAUPAN005354000
  • symbol: SA1885
  • pan gene symbol?: cshA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1885 [new locus tag: SA_RS10845 ]
  • symbol: SA1885
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2135902..2137422
  • length: 1521
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    TTGCAAAATTTTAAAGAACTAGGGATTTCGGATAATACGGTTCAGTCACTTGAATCAATG
    GGATTTAAAGAGCCGACACCTATCCAAAAAGACAGTATCCCTTATGCGTTACAAGGAATT
    GATATCCTTGGGCAAGCTCAAACCGGTACAGGTAAAACAGGAGCATTCGGTATTCCTTTA
    ATTGAGAAAGTAGTAGGGAAACAAGGGGTTCAATCGTTGATTTTAGCACCTACAAGAGAA
    TTGGCAATGCAGGTAGCTGAACAATTAAGAGAATTTAGCCGTGGACAAGGTGTCCAAGTT
    GTTACTGTATTCGGTGGTATGCCTATCGAACGCCAAATTAAAGCCTTGAAAAAAGGCCCA
    CAAATCGTAGTCGGAACACCTGGGCGTGTTATCGACCATTTAAATCGTCGCACATTAAAA
    ACGGACGGAATTCATACTTTGATTTTAGATGAAGCTGATGAAATGATGAATATGGGATTC
    ATCGATGATATGAGATTTATTATGGATAAAATTCCAGCAGTACAACGTCAAACAATGTTG
    TTCTCAGCTACAATGCCTAAAGCAATCCAAGCTTTAGTACAACAATTTATGAAATCACCA
    AAAATCATTAAGACAATGAATAATGAAATGTCTGATCCACAAATCGAAGAATTCTATACA
    ATTGTTAAAGAATTAGAGAAATTTGATACATTTACAAATTTCCTAGATGTTCATCAACCT
    GAATTAGCAATCGTATTCGGACGTACAAAACGTCGTGTTGATGAATTAACAAGTGCTTTG
    ATTTCTAAAGGATATAAAGCTGAAGGCTTACATGGTGATATTACACAAGCGAAACGTTTA
    GAAGTATTAAAGAAATTTAAAAATGACCAAATTAATATTTTAGTCGCTACTGATGTAGCA
    GCAAGAGGACTAGATATTTCTGGTGTGAGTCATGTTTATAACTTTGATATACCTCAAGAT
    ACTGAAAGCTATACACACCGTATTGGTCGTACGGGTCGTGCTGGTAAAGAAGGTATCGCT
    GTAACGTTTGTTAATCCAATCGAAATGGATTATATCAGACAAATTGAAGATGCAAACGGT
    AGAAAAATGAGTGCACTTCGTCCACCTCATCGTAAAGAAGTACTTCAAGCACGTGAAGAT
    GACATCAAAGAAAAAGTTGAAAACTGGATGTCTAAAGAGTCAGAATCACGCTTGAAACGC
    ATTTCTACAGAGTTGTTAAATGAATATAACGATGTTGATTTAGTTGCTGCACTTTTACAA
    GAGTTAGTAGAAGCGAACGATGAAGTTGAAGTTCAATTAACTTTTGAAAAACCATTATCT
    CGCAAAGGCCGTAATGGTAAACCAAGCGGTTCTCGTAACAGAAATAGTAAGCGTGGTAAT
    CCTAAATTTGACAGTAAGAGTAAACGTTCAAAAGGATACTCAAGTAAGAAGAAAAGTACA
    AAAAAATTCGACCGTAAAGAGAAGAGCAGCGGTGGAAGCAGACCTATGAAAGGTCGCACA
    TTTGCTGACCATCAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1521

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1885 [new locus tag: SA_RS10845 ]
  • symbol: SA1885
  • description: hypothetical protein
  • length: 506
  • theoretical pI: 10.144
  • theoretical MW: 56942
  • GRAVY: -0.578854

Function[edit source | edit]

  • reaction:
    EC 3.6.4.13?  ExPASy
    RNA helicaseATP + H2O = ADP + phosphate
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 87)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 75.2)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 54.8)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 46)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 41.7)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 36.7)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairreverse gyrase (TIGR01054; EC 3.6.4.12,5.99.1.3; HMM-score: 31.3)
    CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 26.6)
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 21.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairprimosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 19.7)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtranscription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 18.4)
    CRISPR-associated helicase Cas3, subtype CYANO (TIGR03158; HMM-score: 12.9)
    Unknown functionEnzymes of unknown specificityATP-dependent helicase HrpB (TIGR01970; EC 3.6.4.-; HMM-score: 10.8)
  • TheSEED:  
    ATP-dependent RNA helicases, bacterial DEAD-box ATP-dependent RNA helicase DeaD (= CshA) (EC 3.6.4.13) 
  • PFAM:
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 169)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 112.7)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 25.9)
    CMS1; U3-containing 90S pre-ribosomal complex subunit (PF14617; HMM-score: 17)
    AAA_30; AAA domain (PF13604; HMM-score: 14.8)
    Flavi_DEAD; Flavivirus DEAD domain (PF07652; HMM-score: 13.4)
    ERCC3_RAD25_C; ERCC3/RAD25/XPB C-terminal helicase (PF16203; HMM-score: 12.2)
    no clan definedINTS5_N; Integrator complex subunit 5 N-terminus (PF14837; HMM-score: 10.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA0562(adh1)alcohol dehydrogenase  [2] (data from MRSA252)
    SA2428(arcA)arginine deiminase  [2] (data from MRSA252)
    SA2427(arcB)ornithine carbamoyltransferase  [2] (data from MRSA252)
    SA1984(asp23)alkaline shock protein 23  [2] (data from MRSA252)
    SA0545(eutD)phosphotransacetylase  [2] (data from MRSA252)
    SA0505(fus)elongation factor G  [2] (data from MRSA252)
    SA0727(gap)glyceraldehyde-3-phosphate dehydrogenase  [2] (data from MRSA252)
    SA1959(glmS)glucosamine--fructose-6-phosphate aminotransferase  [2] (data from MRSA252)
    SA1342(gnd)6-phosphogluconate dehydrogenase  [2] (data from MRSA252)
    SA1305(hu)DNA-binding protein II  [2] (data from MRSA252)
    SA0232(lctE)L-lactate dehydrogenase  [2] (data from MRSA252)
    SA2400(mqo2)malate:quinone oxidoreductase  [2] (data from MRSA252)
    SA1938(pdp)pyrimidine-nucleoside phosphorylase  [2] (data from MRSA252)
    SA0496(rplA)50S ribosomal protein L1  [2] (data from MRSA252)
    SA2044(rplB)50S ribosomal protein L2  [2] (data from MRSA252)
    SA2047(rplC)50S ribosomal protein L3  [2] (data from MRSA252)
    SA2046(rplD)50S ribosomal protein L4  [2] (data from MRSA252)
    SA2035(rplE)50S ribosomal protein L5  [2] (data from MRSA252)
    SA2033(rplF)50S ribosomal protein L6  [2] (data from MRSA252)
    SA0497(rplJ)50S ribosomal protein L10  [2] (data from MRSA252)
    SA0495(rplK)50S ribosomal protein L11  [2] (data from MRSA252)
    SA2037(rplN)50S ribosomal protein L14  [2] (data from MRSA252)
    SA2029(rplO)50S ribosomal protein L15  [2] (data from MRSA252)
    SA2022(rplQ)50S ribosomal protein L17  [2] (data from MRSA252)
    SA1084(rplS)50S ribosomal protein L19  [2] (data from MRSA252)
    SA1473(rplU)50S ribosomal protein L21  [2] (data from MRSA252)
    SA2042(rplV)50S ribosomal protein L22  [2] (data from MRSA252)
    SA2045(rplW)50S ribosomal protein L23  [2] (data from MRSA252)
    SA1922(rpmE2)50S ribosomal protein L31  [2] (data from MRSA252)
    SA0500(rpoB)DNA-directed RNA polymerase subunit beta  [2] (data from MRSA252)
    SA2041(rpsC)30S ribosomal protein S3  [2] (data from MRSA252)
    SAS052(rpsD)30S ribosomal protein S4  [2] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [2] (data from MRSA252)
    SA2024(rpsK)30S ribosomal protein S11  [2] (data from MRSA252)
    SA2025(rpsM)30S ribosomal protein S13  [2] (data from MRSA252)
    SA2038(rpsQ)30S ribosomal protein S17  [2] (data from MRSA252)
    SA1499(tig)trigger factor  [2] (data from MRSA252)
    SA1100(tsf)elongation factor Ts  [2] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [2] (data from MRSA252)
    SA0295hypothetical protein  [2] (data from MRSA252)
    SA0940hypothetical protein  [2] (data from MRSA252)
    SA1118hypothetical protein  [2] (data from MRSA252)
    SA1528hypothetical protein  [2] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.153
    • Ymax_pos: 31
    • Cmax: 0.21
    • Cmax_pos: 31
    • Smax: 0.142
    • Smax_pos: 30
    • Smean: 0.098
    • D: 0.131
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILGQAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVTVFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGFIDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEFYTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQAKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRAGKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSKESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSGSRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol. Microbiol.: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] (P p)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 2.35 2.36 2.37 2.38 2.39 2.40 2.41 2.42 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]