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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08535 [old locus tag: SACOL1673 ]
  • pan locus tag?: SAUPAN004215000
  • symbol: SACOL_RS08535
  • pan gene symbol?: alaS
  • synonym:
  • product: alanine--tRNA ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08535 [old locus tag: SACOL1673 ]
  • symbol: SACOL_RS08535
  • product: alanine--tRNA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1699219..1701849
  • length: 2631
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1699219..1701849) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    ATGAAAAAGTTAAAAGCGAGTGAAATTAGACAAAAATATCTAGATTTCTTTGTTGAAAAA
    GGACATATGGTTGAACCTTCTGCACCATTAGTGCCAATTGATGATGATACATTATTATGG
    ATTAATTCAGGTGTAGCAACATTAAAGAAATATTTTGATGGACGTGAAACACCTAAAAAG
    CCAAGAATTGTAAACTCTCAAAAAGCTATTCGTACAAATGATATTGAAAATGTTGGTTTC
    ACAGCGCGTCACCATACATTCTTTGAAATGTTAGGTAACTTCTCAATTGGTGATTATTTT
    AAACAAGAAGCGATTGAATTTGCTTGGGAATTTTTAACGAGTGATAAATGGATGGGTATG
    GAGCCAGATAAATTGTACGTTACGATTCATCCGGAAGATATGGAAGCATACAACATTTGG
    CATAAAGATATTGGGCTTGAAGAAAGTCGTATTATTCGCATTGAAGGTAACTTCTGGGAT
    ATTGGTGAAGGGCCTTCAGGACCGAACACTGAGATTTTCTATGATCGCGGAGAAGCATAT
    GGACAAGACGATCCGGCAGAAGAAATGTATCCAGGTGGAGAAAATGAACGCTATCTTGAA
    GTATGGAACTTAGTATTTAGTGAATTCAATCATAATAAAGATCATAGTTACACACCATTA
    CCTAATAAAAATATTGATACTGGCATGGGGCTTGAGCGTATGGCCTCAGTTTCTCAAAAT
    GTACGTACTAACTATGAAACAGATTTATTTATGCCTATAATGAATGAAATCGAAAAAGTA
    TCAGGTAAACAATATTTAGTAAACAACGAACAAGATGTGGCATTTAAAGTAATTGCTGAC
    CACATTCGTACGATTGCATTTGCAATTTCTGATGGTGCATTACCTGCCAATGAAGGTAGA
    GGGTATGTATTACGTCGATTGTTACGTCGTGCCGTTCGTTTTAGTCAAACGTTAGGAATC
    AATGAGCCATTTATGTACAAACTTGTTGATATTGTTGCAGACATTATGGAACCATATTAT
    CCAAATGTTAAGGAAAAAGCAGATTTCATTAAGCGTGTTATAAAGTCTGAAGAAGAACGA
    TTCCATGAAACATTAGAAGATGGTTTAGCGATTTTAAATGAATTAATTAAAAAAGCTAAA
    GCGACAACAAATGAAATTAATGGGAAAGATGCATTTAAATTGTATGATACGTATGGGTTC
    CCAATTGAATTAACTGAAGAAATAGCAGTGCAAGCAGGATTGAAAGTTGATATGACAACA
    TTCGAGTCAGAAATGCAACAACAACGTGATCGTGCACGTCAAGCACGTCAAAATTCTCAA
    TCAATGCAAGTTCAAAGTGAAGTATTGAAAAATATTACATCTGCAAGTACTTTTGTTGGT
    TATGATACTGCGACAGCTCAAACAACACTAACACACTTGATATACAATGGTGAAGAAGTT
    TCACAAGTTGAAGCGGGTGAAACAGTATACTTCATGTTAACGGAAACACCATTTTATGCA
    ATCAGTGGTGGACAAGTTGCGGATACAGGTATTGTTTATAATGACAATTTTGAAATTGCT
    GTTAGTGAAGTAACCAAAGCACCAAATGGTCAAAACTTGCATAAAGGAGTAGTACAATTT
    GGCCAAGTAAATGTTGGCGCTACAGTGTCTGCTGAAGTGAACCAAAATGATCGACGTGAC
    ATTCAAAAGAACCATAGTGCAACACATTTATTACATGCAGCGTTGAAATCAGTACTGGGT
    GATCATGTTAACCAAGCTGGTTCACTAGTAGAAGCAGATCGTTTACGTTTTGATTTCTCT
    CATTTTGGTCCAATGACTAATGATGAAATTGATCAAGTTGAACGCTTAGTAAATGAAGAA
    ATTTGGAAAGGTATTGACGTTAACATTCAAGAAATGGATATTGCTTCAGCTAAAGAAATG
    GGCGCAATGGCATTATTCGGTGAAAAATATGGTGATGTTGTGCGTGTAGTAAATATGGCA
    CCATTTTCAATTGAATTATGTGGTGGTATTCATGTCCGCAATACTTCTGAAATTGGCTTA
    TTCAAAATAGTAAGTGAGTCAGGTACAGGAGCTGGTGTGCGTCGTATTGAAGCATTAACA
    GGTAAAGCAGCTTTCTTATATTTAGAAGATATTCAAGAGAAATTTAATACGATGAAATCA
    CAGCTGAAAGTGAAATCTGATGATCAAGTAGTCGATAAGTTAACACAATTACAAGATGAA
    GAAAAAGCATTATTAAAACAATTAGAGCAACGTGACAAAGAAATCACATCACTTAAAATG
    GGTAATATTGAAGATCAAGTTGAAGAAATCAATGGCTATAAAGTATTGGTTACTGAAGTG
    GATGTACCAAATGCGAAAGCAATTCGCTCGACAATGGACGATTTTAAATCTAAACTACAA
    GATACAATTATCATTCTTGCAAGTAATGTTGATGATAAAGTATCGATGGTTGCAACTGTC
    CCTAAATCTTTAACAAATAACGTTAAAGCCGGTGATCTTATCAAACAAATGGCACCAATC
    GTTGGTGGTAAAGGTGGCGGTCGTCCAGATATGGCTCAAGGTGGCGGTACACAACCTGAA
    AATATCTCAAAATCATTAAGCTTTATTAAAGATTACATTAAAAATCTATAA
    60
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    2631

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS08535 [old locus tag: SACOL1673 ]
  • symbol: SACOL_RS08535
  • description: alanine--tRNA ligase
  • length: 876
  • theoretical pI: 4.75077
  • theoretical MW: 98519.6
  • GRAVY: -0.418379

Function[edit source | edit]

  • reaction:
    EC 6.1.1.7?  ExPASy
    Alanine--tRNA ligaseATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationalanine--tRNA ligase (TIGR00344; EC 6.1.1.7; HMM-score: 1065.1)
    alanine--tRNA ligase (TIGR03683; EC 6.1.1.7; HMM-score: 421.9)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesclan AA aspartic protease, TIGR02281 family (TIGR02281; EC 3.4.23.-; HMM-score: 12.1)
  • TheSEED:
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2c; tRNA synthetases class II (A) (PF01411; HMM-score: 696.1)
    no clan definedDHHA1; DHHA1 domain (PF02272; HMM-score: 107.2)
    Peptidase_ME (CL0094) tRNA_SAD; Threonyl and Alanyl tRNA synthetase second additional domain (PF07973; HMM-score: 65)
    no clan definedSwm2; Nucleolar protein Swm2 (PF17083; HMM-score: 14.8)
    FAM76; FAM76 protein (PF16046; HMM-score: 9.7)
    CCDC24; Coiled-coil domain-containing protein 24 family (PF15669; HMM-score: 8.9)
    IncA; IncA protein (PF04156; HMM-score: 6.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:
    SACOL_RS11135(deoA)pyrimidine-nucleoside phosphorylase  [1] (data from MRSA252)
    SACOL_RS0303050S ribosomal protein L1  [1] (data from MRSA252)
    SACOL_RS0304050S ribosomal protein L7/L12  [1] (data from MRSA252)
    SACOL_RS03080elongation factor Tu  [1] (data from MRSA252)
    SACOL_RS05630pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SACOL_RS05640dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex  [1] (data from MRSA252)
    SACOL_RS07375hypothetical protein  [1] (data from MRSA252)
    SACOL_RS07385dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)
    SACOL_RS08905isocitrate dehydrogenase (NADP(+))  [1] (data from MRSA252)
    SACOL_RS08930pyruvate kinase  [1] (data from MRSA252)
    SACOL_RS08995universal stress protein UspA  [1] (data from MRSA252)
    SACOL_RS1169530S ribosomal protein S5  [1] (data from MRSA252)
    SACOL_RS13725malate:quinone oxidoreductase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.125
    • Ymax_pos: 11
    • Cmax: 0.106
    • Cmax_pos: 7
    • Smax: 0.183
    • Smax_pos: 1
    • Smean: 0.15
    • D: 0.135
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446656718 NCBI
  • RefSeq: WP_000734064 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSGVATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQEAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWDIGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYTPLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFKVIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVADIMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAFKLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLKNITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVADTGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHSATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKGIDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFKIVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDEEKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSKLQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGGTQPENISKSLSFIKDYIKNL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08535 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]