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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS09265 [old locus tag: SACOL1808 ]
  • pan locus tag?: SAUPAN004441000
  • symbol: SACOL_RS09265
  • pan gene symbol?: leuS
  • synonym:
  • product: leucine--tRNA ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS09265 [old locus tag: SACOL1808 ]
  • symbol: SACOL_RS09265
  • product: leucine--tRNA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1862816..1865230
  • length: 2415
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1862816..1865230) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
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    361
    421
    481
    541
    601
    661
    721
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    841
    901
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    1021
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    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    TTGAATTACAACCACAATCAAATTGAAAAGAAATGGCAAGACTATTGGGACGAAAATAAA
    ACATTTAAAACAAATGATAACTTAGGTCAAAAGAAATTTTATGCTTTAGACATGTTTCCA
    TATCCATCAGGTGCTGGTTTACATGTTGGACATCCTGAGGGCTATACAGCAACAGATATC
    ATTTCAAGATATAAAAGAATGCAAGGATATAATGTATTACATCCGATGGGGTGGGATGCA
    TTCGGATTACCAGCAGAGCAATATGCTTTAGACACTGGCAACGACCCACGTGAATTTACA
    AAGAAAAATATCCAAACTTTTAAACGACAAATTAAAGAATTAGGGTTCAGTTATGATTGG
    GATCGTGAAGTTAATACAACAGATCCAGAATACTATAAATGGACACAGTGGATTTTCATA
    CAGTTATATAACAAAGGTTTAGCATACGTTGATGAAGTTGCAGTTAACTGGTGTCCAGCA
    TTAGGCACTGTTTTATCTAACGAAGAAGTGATTGATGGTGTCTCTGAACGTGGTGGACAT
    CCAGTTTATCGTAAGCCGATGAAACAATGGGTACTTAAAATCACAGAATATGCAGATCAA
    TTATTAGCAGATTTAGATGATTTAGATTGGCCTGAGTCTTTAAAAGATATGCAGCGCAAT
    TGGATTGGACGTTCTGAAGGGGCCAAAGTTTCATTTGATGTAGATAATACGGAAGGAAAA
    GTAGAAGTATTTACGACTAGACCAGATACAATCTATGGTGCATCATTCTTAGTCTTAAGT
    CCTGAACATGCATTAGTTAATTCAATTACAACAGATGAATATAAAGAAAAAGTAAAAGCT
    TATCAAACAGAAGCTTCTAAAAAGTCAGATTTAGAACGTACAGATTTAGCAAAAGATAAA
    TCAGGTGTATTTACTGGTGCATATGCAACTAATCCTTTATCTGGTGAAAAAGTACAAATT
    TGGATTGCTGATTATGTATTATCAACATATGGTACTGGAGCAATTATGGCAGTACCAGCG
    CATGATGACAGAGATTATGAATTTGCTAAAAAGTTTGATTTGCCAATCATTGAAGTCATC
    GAAGGTGGAAATGTTGAAGAAGCAGCATACACTGGTGAAGGTAAACATATTAATTCTGGT
    GAACTTGATGGTTTAGAAAATGAAGCGGCAATTACTAAAGCTATTCAATTATTAGAGCAA
    AAAGGTGCTGGCGAAAAGAAAGTTAATTACAAATTAAGAGATTGGTTATTCAGTCGTCAG
    CGTTATTGGGGCGAACCAATTCCTGTCATTCATTGGGAAGATGGAACAATGACAACTGTT
    CCTGAAGAAGAGCTACCATTGTTGTTACCTGAAACAGATGAAATCAAGCCATCAGGGACT
    GGTGAGTCTCCACTAGCTAATATTGATTCATTTGTAAATGTTGTAGATGAAAAAACAGGT
    ATGAAAGGACGTCGTGAAACAAATACAATGCCACAATGGGCAGGTAGTTGTTGGTATTAT
    TTACGTTACATCGATCCTAAAAATGAAAATATGTTAGCAGATCCTGAAAAATTAAAACAT
    TGGTTACCTGTTGATTTATATATCGGTGGAGTAGAACATGCGGTTCTTCACTTATTATAT
    GCAAGATTTTGGCATAAAGTCCTTTATGATTTGGCTATCGTACCTACTAAAGAACCTTTC
    CAAAAATTATTTAACCAAGGTATGATTTTAGGAGAAGGTAATGAGAAGATGAGTAAATCT
    AAAGGAAATGTAATCAATCCTGATGATATAGTACAGTCTCATGGTGCAGATACTTTGCGT
    CTTTACGAAATGTTTATGGGACCTTTAGATGCTGCAATTGCATGGAGTGAAAAAGGATTA
    GATGGGTCTCGTCGATTCTTAGATCGCGTATGGCGTTTAATGGTAAATGAAGATGGGACA
    TTGAGTTCAAAAATTGTAACTACAAATAATAAATCTTTAGATAAAGTTTATAACCAAACT
    GTTAAAAAGGTAACAGAAGACTTTGAAACATTAGGATTTAATACTGCTATTAGTCAATTA
    ATGGTATTTATTAATGAGTGTTATAAAGTTGATGAAGTTTATAAACCTTACATTGAAGGC
    TTCGTTAAAATGTTAGCACCTATTGCACCACATATCGGTGAAGAATTATGGTCAAAATTA
    GGACATGAAGAGTCTATTACGTACCAACCTTGGCCAACTTATGACGAAGCACTACTTGTA
    GATGATGAAGTAGAAATCGTTGTTCAAGTGAATGGTAAATTGAGAGCTAAAATTAAAATT
    GCTAAAGATACATCAAAAGAAGAAATGCAAGAAATTGCCTTATCTAATGACAATGTTAAA
    GCGAGTATTGAAGGTAAAGACATCATGAAAGTCATCGCTGTTCCTCAAAAATTAGTCAAT
    ATTGTAGCTAAATAA
    60
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    2160
    2220
    2280
    2340
    2400
    2415

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS09265 [old locus tag: SACOL1808 ]
  • symbol: SACOL_RS09265
  • description: leucine--tRNA ligase
  • length: 804
  • theoretical pI: 4.78881
  • theoretical MW: 91672.1
  • GRAVY: -0.503856

Function[edit source | edit]

  • reaction:
    EC 6.1.1.4?  ExPASy
    Leucine--tRNA ligaseATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 1186.6)
    Genetic information processingProtein synthesistRNA aminoacylationvaline--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 310.1)
    Genetic information processingProtein synthesistRNA aminoacylationisoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 229.7)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 227.8)
    Genetic information processingProtein synthesistRNA aminoacylationmethionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 114.3)
    Genetic information processingProtein synthesistRNA aminoacylationcysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 37.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogscysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 26.2)
  • TheSEED:
  • PFAM:
    HUP (CL0039) tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 245.5)
    tRNA-synt_1_2; Leucyl-tRNA synthetase, Domain 2 (PF13603; HMM-score: 227)
    tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 96.8)
    DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA (PF08264; HMM-score: 82.7)
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 25.9)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 18.3)
    tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.111
    • Ymax_pos: 56
    • Cmax: 0.103
    • Cmax_pos: 58
    • Smax: 0.178
    • Smax_pos: 45
    • Smean: 0.097
    • D: 0.106
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447031476 NCBI
  • RefSeq: WP_001108732 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCPALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQKLVNIVAK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS09265 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]