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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS13990 [old locus tag: SACOL2671 ]
  • pan locus tag?: SAUPAN006382000
  • symbol: SACOL_RS13990
  • pan gene symbol?: secA2
  • synonym:
  • product: accessory Sec system translocase SecA2

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS13990 [old locus tag: SACOL2671 ]
  • symbol: SACOL_RS13990
  • product: accessory Sec system translocase SecA2
  • replicon: chromosome
  • strand: -
  • coordinates: 2740325..2742715
  • length: 2391
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2740325..2742715) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    481
    541
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    841
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    1021
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    2341
    ATGAAACATAAACTAGATGTTACGATAAACGAATTAAGACTAAAGTCGATTAGAAAAATA
    GTGAAACGCATCAATACATGGAGCGATGAAGTTAAATCATATTCTGATGATGCGTTAAAA
    CAAAAGACAATAGAATTTAAAGAACGTTTAGCATCAGGAGTTGATACACTAGATACATTG
    TTACCTGAAGCTTATGCAGTGGCACGTGAAGCGAGCTGGAGAGTATTAGGTATGTATCCT
    AAAGAAGTTCAGTTAATCGGAGCGATTGTATTGCATGAAGGTAACATTGCGGAGATGCAA
    ACAGGCGAAGGTAAAACATTAACGGCAACGATGCCTTTATATTTAAATGCTCTTTCGGGA
    AAAGGAACATATTTAATTACAACGAATGATTATTTAGCGAAGCGAGATTTTGAAGAGATG
    CAACCATTATATGAATGGTTAGGTTTAACTGCTTCATTAGGTTTTGTTGATATTGTGGAT
    TATGAGTATCAAAAAGGGGAAAAACGTAATATATATGAACATGATATTATATATACAACT
    AATGGACGTCTCGGTTTTGATTATTTAATTGATAATTTGGCTGATAGTGCGGAAGGGAAA
    TTTTTACCACAATTAAATTACGGTATTATTGATGAAGTGGATTCAATCATTTTAGATGCT
    GCTCAAACACCATTAGTTATTTCGGGTGCACCAAGATTACAATCAAATCTATTTCATATT
    GTGAAAGAGTTTGTAGATACATTGATTGAAGACGTGCATTTTAAAATGAAGAAGACCAAA
    AAAGAAATATGGCTGTTAAATCAAGGTATTGAAGCGGCACAATCATACTTTAATGTTGAA
    GATTTATATAGCGAACAAGCGATGGTCCTAGTGCGTAATATTAATTTAGCACTGCGCGCA
    CAATATTTGTTTGAATCTAATGTCGATTACTTTGTATATAATGGTGATATTGTTTTAATT
    GACCGTATTACAGGTCGTATGTTACCGGGAACTAAGTTGCAAGCTGGACTTCACCAAGCT
    ATTGAAGCGAAAGAAGGTATGGAGGTTTCAACAGATAAAAGTGTTATGGCAACAATTACC
    TTCCAGAATTTATTTAAACTTTTTGAATCATTTTCAGGTATGACAGCTACAGGAAAATTA
    GGCGAATCAGAGTTCTTTGATTTGTATTCAAAAATAGTCGTACAAGTACCAACTGATAAA
    GCGATTCAACGTATCGATGAACCAGATAAAGTGTTTCGTTCAGTTGATGAGAAAAACATC
    GCGATGATTCATGATATAGTTGAACTTCATGAAACGGGGCGACCGGTTTTACTCATAACG
    AGAACTGCTGAAGCGGCTGAATACTTTTCGAAAGTATTATTCCAAATGGATATTCCTAAT
    AATTTACTCATTGCGCAAAATGTTGCAAAAGAAGCGCAGATGATAGCTGAAGCAGGGCAA
    ATTGGTTCCATGACTGTTGCGACTAGTATGGCAGGTCGAGGCACAGATATTAAACTTGGT
    GAAGGTGTCGAAGCATTAGGTGGATTAGCTGTTATTATTCATGAACATATGGAAAATAGC
    CGTGTAGACAGGCAATTACGTGGTCGTTCTGGTAGACAAGGGGATCCGGGATCATCTTGT
    ATATATATTTCACTAGATGATTATTTAGTTAAGCGATGGAGCGATAGTAATTTAGCGGAA
    AATAATCAATTATATTCATTAGATGCACAACGATTATCGCAAAGTAATTTGTTTAATCGC
    AAAGTTAAGCAAATTGTAGTTAAAGCGCAGCGTATCTCGGAAGAACAAGGGGTTAAAGCT
    CGTGAAATGGCTAATGAATTTGAAAAAAGCATTAGTATACAGCGAGATCTTGTATACGAG
    GAACGCAATCGAGTTTTAGAAATAGATGATGCTGAGAATCAAGATTTTAAAGCGTTAGCT
    AAAGATGTATTTGAAATGTTTGTAAATGAGGAAAAGGTGCTAACAAAATCGCGTGTTGTC
    GAGTATATTTATCAAAATTTAAGTTTCCAATTTAATAAAGATGTGGCTTGTGTTAATTTT
    AAAGATAAGCAAGCAGTAGTGACATTTTTATTAGAGCAATTTGAAAAGCAATTAGCTTTG
    AATCGTAAAAACATGCAAAGTGCATATTATTATAATATTTTCGTTCAAAAAGTCTTTTTG
    AAAGCAATTGATTCATGTTGGTTAGAACAAGTCGACTATTTACAACAATTAAAAGCAAGC
    GTTAATCAACGACAAAATGGGCAACGTAACGCTATTTTTGAATACCATAGAGTTGCCTTA
    GATTCATTTGAAGTTATGACACGAAATATCAAAAAAAGAATGGTGAAAAATATTTGTCAA
    AGCATGATTACATTTGATAAAGAGGGTATGCCAGTTATACATTTTCCATAA
    60
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    2160
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    2391

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS13990 [old locus tag: SACOL2671 ]
  • symbol: SACOL_RS13990
  • description: accessory Sec system translocase SecA2
  • length: 796
  • theoretical pI: 5.29414
  • theoretical MW: 90929.4
  • GRAVY: -0.265704

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein and peptide secretion and traffickingaccessory Sec system translocase SecA2 (TIGR03714; HMM-score: 1241.3)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingpreprotein translocase, SecA subunit (TIGR00963; HMM-score: 689.5)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingaccessory Sec system translocase SecA2 (TIGR04397; HMM-score: 668.9)
    accessory Sec system translocase SecA2 (TIGR04221; HMM-score: 484.6)
  • TheSEED:
  • PFAM:
    P-loop_NTPase (CL0023) SecA_DEAD; SecA DEAD-like domain (PF07517; HMM-score: 340.6)
    no clan definedSecA_PP_bind; SecA preprotein cross-linking domain (PF01043; HMM-score: 91.1)
    SecA_SW; SecA Wing and Scaffold domain (PF07516; HMM-score: 88.1)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 27.7)
    no clan definedMrx7; MIOREX complex component 7 (PF10906; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.146
    • Ymax_pos: 14
    • Cmax: 0.109
    • Cmax_pos: 69
    • Smax: 0.267
    • Smax_pos: 4
    • Smean: 0.215
    • D: 0.173
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446603601 NCBI
  • RefSeq: WP_000680947 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTIEFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTGEGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVDYEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIILDAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSYFNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQAGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKIVVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFSKVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGGLAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYSLDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNRVLEIDDAENQDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKDKQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKASVNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS13990 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]