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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS11775 [old locus tag: SACOL2238 ]
  • pan locus tag?: SAUPAN005701000
  • symbol: SACOL_RS11775
  • pan gene symbol?: rplD
  • synonym:
  • product: 50S ribosomal protein L4

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS11775 [old locus tag: SACOL2238 ]
  • symbol: SACOL_RS11775
  • product: 50S ribosomal protein L4
  • replicon: chromosome
  • strand: -
  • coordinates: 2305838..2306461
  • length: 624
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (2305838..2306461) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCTAATTATGATGTTTTAAAATTAGACGGAACTAAATCAGGTTCAATCGAATTAAGC
    GATGCAGTATTCGGTATTGAGCCAAATAATAGCGTTTTATTCGAAGCTATTAATTTACAA
    CGTGCTTCATTACGTCAAGGTACGCATGCTGTTAAGAATCGTTCAGCAGTAAGCGGTGGC
    GGACGTAAACCATGGAAGCAAAAAGGAACAGGTCGTGCTCGTCAAGGTACAATCCGTGCT
    CCACAATGGCGTGGCGGTGGTATCGTATTCGGACCAACTCCAAGAAGTTATGCATACAAA
    ATGCCTAAGAAAATGCGTCGTTTAGCTTTACGCTCAGCATTATCTTTCAAAGCTCAAGAG
    AATGGCTTAACTGTAGTTGACGCATTCAACTTCGAAGCTCCAAAAACTAAAGAATTCAAA
    AATGTATTATCTACATTAGAACAACCTAAAAAAGTATTAGTAGTTACTGAAAACGAAGAT
    GTAAATGTTGAATTATCAGCACGCAACATCCCTGGCGTTCAAGTGACAACTGCTCAAGGT
    TTAAATGTTTTAGATATCACTAATGCTGACAGCTTAGTAATTACTGAAGCTGCTGCTAAA
    AAAGTTGAGGAGGTGCTCGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    624

Protein[edit source | edit]

Protein Data Bank: 5LI0
Protein Data Bank: 4WCE
Protein Data Bank: 4WFB
Protein Data Bank: 4WF9
Protein Data Bank: 4WFA
Protein Data Bank: 5HL7

General[edit source | edit]

  • locus tag: SACOL_RS11775 [old locus tag: SACOL2238 ]
  • symbol: SACOL_RS11775
  • description: 50S ribosomal protein L4
  • length: 207
  • theoretical pI: 10.5993
  • theoretical MW: 22464.5
  • GRAVY: -0.348309

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modification50S ribosomal protein uL4 (TIGR03953; HMM-score: 272.5)
    50S ribosomal protein uL4 (TIGR03672; HMM-score: 38.3)
  • TheSEED:
  • PFAM:
    no clan definedRibosomal_L4; Ribosomal protein L4/L1 family (PF00573; HMM-score: 240.6)
    DHHA1; DHHA1 domain (PF02272; HMM-score: 15.6)
    Concanavalin (CL0004) Reoviridae_Vp9; Reoviridae VP9 (PF08978; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.133
    • Ymax_pos: 51
    • Cmax: 0.136
    • Cmax_pos: 23
    • Smax: 0.208
    • Smax_pos: 50
    • Smean: 0.121
    • D: 0.129
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 445946972 NCBI
  • RefSeq: WP_000024827 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASLRQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMPKKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENEDVNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS11775 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 1.108 1.109 1.110 1.111 1.112 1.113 1.114 1.115 1.116 1.117 1.118 1.119 1.120 1.121 1.122 1.123 1.124 1.125 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]