From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2028 [new locus tag: SA_RS11665 ]
  • pan locus tag?: SAUPAN005682000
  • symbol: secY
  • pan gene symbol?: secY
  • synonym:
  • product: preprotein translocase subunit SecY

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2028 [new locus tag: SA_RS11665 ]
  • symbol: secY
  • product: preprotein translocase subunit SecY
  • replicon: chromosome
  • strand: -
  • coordinates: 2298151..2299443
  • length: 1293
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATTCAAACCCTTGTGAACTTCTTTAGAACAAAAGAGGTTCGTAACAAGATTTTCTTC
    ACACTAGCAATGTTAGTAATTTTTAAAATAGGGACTTATATACCAGCTCCAGGAGTAAAT
    CCTGCAGCTTTTGATAATCCCCAAGGTTCTCAAGGTGCCACTGAGTTATTAAATACTTTT
    GGTGGCGGAGCCTTGAAACGATTTTCTATTTTTGCAATGGGTATTGTACCCTACATCACT
    GCATCAATCGTAATGCAATTATTACAAATGGATATTGTCCCTAAATTCTCAGAATGGGCA
    AAACAAGGTGAAGTAGGTAGAAGAAAGTTAAATAACGTTACTCGTTATTTAGCAATTTCT
    TTAGCATTTATCCAATCTATAGGTATGGCATTCCAATTTAATAATTATCTCAAAGGTGCG
    CTGATTATCAATCAGTCAATTATGAGTTATTTATTAATAGCACTAGTTTTGACAGCAGGA
    ACTGCTTTCTTAATATGGCTTGGTGATCAAATCACTCAGTTCGGTGTTGGTAATGGTATT
    TCTATTATCATATTTGCGGGTATTTTATCAACGTTGCCAGCCAGTCTAATTCAATTTGGA
    CAAACTGCGTTCGTTGGGCAAGAAGATACATCATTAGCATGGTTGAAAGTATTAGGACTT
    TTAGTGTCACTGATACTATTAACTGTAGGTGCTATTTATGTACTTGAAGCCGTTCGTAAA
    ATTCCGATTCAATATGCTAAGAAACAAACTGCTCAAAGATTAGGTTCACAGGCAACTTAT
    CTACCTTTAAAAGTGAACTCAGCCGGTGTTATTCCTGTAATCTTTGCAATGGCGTTCTTC
    TTACTGCCTAGAACATTAACGTTGTTCTATCCAGATAAAGAATGGGCACAAAACATTGCG
    AATGCTGCAAATCCTTCAAGTAATGTTGGTATGGTTGTATATATTGTACTAATTATACTA
    TTCACATACTTCTATGCATTTGTACAAGTTAATCCGGAAAAAATGGCTGATAACCTTAAG
    AAACAAGGTAGCTATGTTCCAGGCATTAGACCTGGTGAACAAACCAAAAAATATATTACT
    AAAGTTTTATATCGTTTAACTTTTGTAGGTTCTATCTTCTTAGCCGTTATTTCAATTCTA
    CCAATATTAGCAACTAAGTTTATGGGATTACCACAATCAATTCAGATTGGTGGTACAAGT
    TTACTTATCGTTATTGGTGTAGCGATTGAAACAATGAAATCTTTAGAAGCTCAAGTATCT
    CAAAAAGAATATAAAGGCTTTGGTGGTAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1293

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2028 [new locus tag: SA_RS11665 ]
  • symbol: SecY
  • description: preprotein translocase subunit SecY
  • length: 430
  • theoretical pI: 10.3709
  • theoretical MW: 47147.6
  • GRAVY: 0.554419

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking preprotein translocase, SecY subunit (TIGR00967; HMM-score: 482.9)
    and 1 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system translocase SecY2 (TIGR02920; HMM-score: 209.7)
  • TheSEED  :
    • Protein translocase subunit SecY
    tRNA synthetase relationships  Preprotein translocase secY subunit (TC 3.A.5.1.1)
  • PFAM:
    no clan defined SecY; SecY translocase (PF00344; HMM-score: 360.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.461739
    • TAT(Tat/SPI): 0.011703
    • LIPO(Sec/SPII): 0.015722
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAFDNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQGEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAGTAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVLGLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFAMAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEKMADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQSIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA2028&oldid=96945"