PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS14320 [old locus tag: SAUSA300_2574 ]
• pan locus tag^?: SAUPAN006370000
• symbol: SAUSA300_RS14320
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS14320 [old locus tag: SAUSA300_2574 ]
• symbol: SAUSA300_RS14320
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2786850..2787308
• length: 459
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2786850..2787308) NCBI
• BioCyc: SAUSA300_RS14320 BioCyc
• MicrobesOnline: see SAUSA300_2574

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  TTGAAAAAACTTCTTATTACCATCGTAATTATCGTATTAATCGGAGCACTTGGGTTCGCA
  ATTTATGCTGCAATAAATCATTCTACATCATCAAGTAATAACCAAGAACAAGAAAATAAA
  TCTACGCATAAAAAGACAGAAACCGAAGAAAATGATCAACAAGATGATAGTGCAGACAAA
  ACAGATAAAAATCAAAATAATGGCAATAACGTTACTAATGACAATCAACCATCTTCACCG
  TCAAATGTTCCTAACAACCATTCAACAGTACCATCGAATACGCAACCAGCTCAGCCAAAA
  GACTCAAATTCAAATAAAAATAATACTCACGGTGGTGGCAGTCAATCGTCAACAAACAAC
  CAAAATAATCAACAACAGAACACACCACCTGCTAATAAATCAAATAATACCAATACGCCT
  TCTAAACAAACACCTCAAGCACAATCACCTAAAAATTAA
  60
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  459
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS14320 [old locus tag: SAUSA300_2574 ]
• symbol: SAUSA300_RS14320
• description: hypothetical protein
• length: 152
• theoretical pI: 7.85378
• theoretical MW: 16361.2
• GRAVY: -1.46579

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Cell division cell division protein ZipA (TIGR02205; HMM-score: 21.3)
  and 10 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transporter family protein (TIGR00860; HMM-score: 15.5)
  Cellular processes Cellular processes Sporulation and germination stage III sporulation protein AG (TIGR02830; HMM-score: 15.5)
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 13.4)
  Metabolism Energy metabolism Electron transport sulfocyanin (TIGR03094; HMM-score: 13)
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions entry exclusion protein TrbK (TIGR04361; HMM-score: 11.9)
  mycoides cluster lipoprotein, LppA/P72 family (TIGR03490; HMM-score: 11.7)
  Cellular processes Cellular processes Pathogenesis putative immunoglobulin-blocking virulence protein (TIGR04526; HMM-score: 8.8)
  two transmembrane protein (TIGR04527; HMM-score: 8.3)
  Metabolism Transport and binding proteins Cations and iron carrying compounds potassium uptake protein, Trk family (TIGR00934; HMM-score: 5.8)
  Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside Transporter (TIGR00939; HMM-score: 5.4)
• TheSEED: see SAUSA300_2574
• PFAM:
  no clan defined DUF4083; Domain of unknown function (DUF4083) (PF13314; HMM-score: 18.6)
  SARAF; SOCE-associated regulatory factor of calcium homoeostasis (PF06682; HMM-score: 16.5)
  and 18 more
  CytochromB561_N; Cytochrome B561, N terminal (PF09786; HMM-score: 14.4)
  DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 14)
  TSPAN_4TM-like (CL0347) DUF4064; Protein of unknown function (DUF4064) (PF13273; HMM-score: 13.8)
  no clan defined UPF0767; UPF0767 family (PF15990; HMM-score: 13.6)
  TSPAN_4TM-like (CL0347) Tetraspanin; Tetraspanin family (PF00335; HMM-score: 12.5)
  no clan defined PsaL; Photosystem I reaction centre subunit XI (PF02605; HMM-score: 12.5)
  Ac76; Orf76 (Ac76) (PF05814; HMM-score: 12.1)
  FeoB_associated; FeoB-associated Cys-rich membrane protein (PF12669; HMM-score: 11.8)
  DMT (CL0184) TMEM144; Transmembrane family, TMEM144 of transporters (PF07857; HMM-score: 10.4)
  Transporter (CL0375) Connexin; Connexin (PF00029; HMM-score: 10.3)
  no clan defined DUF6520; Family of unknown function (DUF6520) (PF20130; HMM-score: 10.2)
  Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 10.1)
  GPCR_A (CL0192) SID-1_RNA_chan; dsRNA-gated channel SID-1 (PF13965; HMM-score: 9.9)
  no clan defined Engrail_1_C_sig; Engrailed homeobox C-terminal signature domain (PF10525; HMM-score: 9.1)
  RBM_T3SS-Flag (CL0873) SpoIIIAH; SpoIIIAH-like protein (PF12685; HMM-score: 7.8)
  no clan defined DUF6080; Family of unknown function (DUF6080) (PF19558; HMM-score: 7.7)
  FAM176; FAM176 family (PF14851; HMM-score: 7.4)
  PFF1_TM; Vacuolar membrane protease, transmembrane domain (PF22251; HMM-score: 6.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.05
  • Cellwall Score: 0.82
  • Extracellular Score: 9.13
  • Internal Helix: 1
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.3505
  • Cell wall & surface Score: 0.0023
  • Extracellular Score: 0.6472
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 23 aa
  • SP(Sec/SPI): 0.895299
  • TAT(Tat/SPI): 0.002039
  • LIPO(Sec/SPII): 0.084029
  • Cleavage Site: CS pos: 23-24. IYA-AI. Pr: 0.4477
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446657457 NCBI
• RefSeq: WP_000734803 NCBI
• UniProt: see SAUSA300_2574

⊟Protein sequence

• MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKSTHKKTETEENDQQDDSADKTDKNQNNGNNVTNDNQPSSPSNVPNNHSTVPSNTQPAQPKDSNSNKNNTHGGGSQSSTNNQNNQQQNTPPANKSNNTNTPSKQTPQAQSPKN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications