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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS07065 [old locus tag: SA1245 ]
  • pan locus tag?: SAUPAN003831000
  • symbol: SA_RS07065
  • pan gene symbol?: sucA
  • synonym:
  • product: 2-oxoglutarate dehydrogenase E1 component

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS07065 [old locus tag: SA1245 ]
  • symbol: SA_RS07065
  • product: 2-oxoglutarate dehydrogenase E1 component
  • replicon: chromosome
  • strand: -
  • coordinates: 1419176..1421908
  • length: 2733
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1419176..1421908) NCBI
  • BioCyc: SA_RS07065 BioCyc
  • MicrobesOnline: see SA1245

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGACTAACGAAAGAAAAGAAGTTTCAGAGGCTCCTGTAAACTTCGGTGCGAATTTAGGT
    CTAATGTTAGATCTATATGATGACTTTTTACAAGATCCATCATCTGTACCAGAAGATTTA
    CAAGTCTTATTCAGCACAATTAAGCGTGTCATGCGTCTAATTGATAATATTCGCCAATAC
    GGGCATCTTAAAGCCGATATTTATCCTGTAAATCCTCCAAAAAGGAAACATGTACCTAAA
    TTAGAGATTGAAGACTTTGATTTAGATCAACAGACTTTGGAAGGTATATCAGCAGGAATT
    GTTTCAGATCACTTTGCCGACATTTATGATAATGCTTATGAAGCAATTTTAAGAATGGAA
    AAACGTTACAAAGGACCAATTGCATTTGAGTATACACATATTAATAACAATACCGAACGT
    GGTTGGTTAAAAAGAAGAATTGAAACGCCATATAAAGTAACGTTAAATAATAACGAAAAA
    AGGGCACTATTCAAACAATTAGCGTATGTTGAAGGGTTTGAAAAATATCTTCATAAAAAC
    TTCGTTGGTGCAAAGCGTTTTTCAATTGAAGGGGTAGACGCACTTGTACCGATGTTACAA
    CGTACTATTACGATTGCTGCGAAAGAAGGTATTAAAAATATACAAATAGGCATGGCTCAC
    CGTGGACGTTTAAACGTTTTAACGCATGTCTTAGAAAAACCGTACGAAATGATGATTTCA
    GAATTTATGCATACAGATCCAATGAAATTCTTACCTGAAGATGGTAGCTTGCAGTTAACT
    GCTGGATGGACTGGTGATGTGAAATATCACCTTGGTGGCATTAAAACTACTGATTCATAC
    GGTACAATGCAGCGTATTGCACTGGCTAACAATCCAAGTCACTTGGAAATTGTTGCACCT
    GTTGTTGAGGGGCGTACGAGAGCAGCACAAGATGATACACAACGAGCTGGGGCTCCGACG
    ACTGATCATCATAAAGCAATGCCAATTATTATACATGGCGATGCTGCTTATCCTGGTCAA
    GGAATTAACTTCGAAACAATGAACTTAGGAAACTTGAAAGGCTATTCTACGGGTGGTTCA
    TTGCATATTATTACTAACAATAGAATTGGATTTACTACAGAACCAATTGATGCACGTTCA
    ACAACTTATTCTACAGATGTGGCCAAAGGTTATGATGTGCCAATATTCCATGTCAATGCT
    GATGACGTTGAAGCTACTATTGAAGCAATTGATATTGCAATGGAATTTAGAAAAGAGTTT
    CATAAAGACGTCGTTATTGATTTAGTAGGTTATCGTCGTTTCGGACATAACGAAATGGAT
    GAACCATCAATTACTAATCCAGTTCCTTATCAGAATATTCGCAAACATGACTCTGTTGAA
    TATGTGTTTGGTAAAAAGCTTGTTAATGAAGGTGTCATTTCAGAAGATGAAATGCATTCA
    TTTATAGAACAAGTCCAAAAGGAACTAAGACAAGCTCATGATAAAATTAATAAAGCTGAT
    AAAATGGATAATCCAGATATGGAAAAACCTGCAGAACTTGCATTACCGTTACAAGCAGAC
    GAACAATCATTTACGTTTGATCATTTGAAAGAAATAAATGATGCATTGTTAACATATCCG
    GATGGCTTTAACATTTTGAAAAAGTTAAACAAAGTTCTTGAGAAGCGTCATGAGCCGTTT
    AATAAAGAAGATGGCTTAGTTGATTGGGCACAAGCAGAACAACTTGCATTTGCGACAATT
    TTACAAGATGGTACACCGATTCGCTTAACTGGTCAAGATAGTGAACGTGGTACATTCAGT
    CATAGGCATGCCGTGTTACATGATGAGCAAACAGGTGAAACATATACACCTTTACATCAT
    GTTCCTGATCAAAAAGCGACATTTGATATACACAATTCTCCGCTTTCAGAAGCAGCAGTA
    GTTGGTTTTGAATACGGCTATAATGTGGAAAACAAAAAAAGCTTCAATATTTGGGAAGCG
    CAATATGGTGATTTTGCAAATATGTCACAAATGATTTTTGACAACTTCTTATTCAGTTCT
    CGCTCAAAATGGGGAGAACGTTCAGGATTAACATTATTCTTACCTCATGCATATGAGGGT
    CAAGGACCTGAACATTCATCAGCAAGATTAGAGCGATTTTTACAATTAGCTGCTGAAAAT
    AATTGCACAGTTGTCAACTTATCTAGTTCAAGTAATTATTTCCACTTATTGCGTGCACAA
    GCAGCTAGTTTAGATTCTGAACAAATGCGACCATTGGTTGTTATGTCACCAAAAAGCTTA
    TTGAGAAATAAAACAGTTGCCAAACCAATTGATGAGTTTACTTCTGGTGGATTTGAGCCA
    ATTTTGACAGAATCATATCAAGCGGATAAGGTTACAAAAGTTATTTTAGCAACTGGTAAA
    ATGTTCATTGATTTAAAAGAAGCATTAGCTAAAAATCCAGACGAATCCGTGTTACTCGTT
    GCGATTGAAAGATTGTATCCATTCCCAGAGGAAGAGATTGAAGCATTACTAGCACAATTG
    CCAAAACTTGAAGAAGTGTCATGGGTACAAGAAGAACCTAAAAATCAAGGTGCATGGTTA
    TATGTCTATCCATATGTTAAAGTGCTAGTTGCAGATAAATATGATTTAAGTTATCATGGC
    AGAATTCAAAGGGCTGCTCCAGCTGAAGGCGATGGAGAAATTCATAAACTTGTTCAAAAT
    AAAATTATAGAAAATGCATTAAAAAATAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2733

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS07065 [old locus tag: SA1245 ]
  • symbol: SA_RS07065
  • description: 2-oxoglutarate dehydrogenase E1 component
  • length: 910
  • theoretical pI: 5.51913
  • theoretical MW: 103111
  • GRAVY: -0.462637

⊟Function[edit | edit source]

  • reaction:
    EC 1.2.4.2?  ExPASy
    Oxoglutarate dehydrogenase (succinyl-transferring) 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
  • TIGRFAM:
    Metabolism Energy metabolism TCA cycle oxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 1026.9)
    and 2 more
    Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 52.7)
    Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 31.3)
  • TheSEED: see SA1245
  • PFAM:
    THDP-binding (CL0254) E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 221.4)
    Transket_pyr; Transketolase, pyrimidine binding domain (PF02779; HMM-score: 180.3)
    and 4 more
    TKC_like (CL0591) OxoGdeHyase_C; 2-oxoglutarate dehydrogenase C-terminal (PF16870; HMM-score: 115.8)
    no clan defined 2-oxogl_dehyd_N; 2-oxoglutarate dehydrogenase N-terminus (PF16078; HMM-score: 20.2)
    B_GA (CL0598) DUF1542; Domain of Unknown Function (DUF1542) (PF07564; HMM-score: 13.2)
    no clan defined DUF1840; Domain of unknown function (DUF1840) (PF08895; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: thiamine diphosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.95
    • Cytoplasmic Membrane Score: 0.05
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001033
    • TAT(Tat/SPI): 0.000223
    • LIPO(Sec/SPII): 0.000383
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLFSTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPKLEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNTERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALVPMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPEDGSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDDTQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIGFTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDLVGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQKELRQAHDKINKADKMDNPDMEKPAELALPLQADEQSFTFDHLKEINDALLTYPDGFNILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHRHAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEAQYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAAENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSGGFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIEALLAQLPKLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDGEIHKLVQNKIIENALKNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS09855(gatA)glutamyl-tRNA(Gln) amidotransferase subunit A  [1] (data from MRSA252)
    SA_RS00310aminoglycoside O-nucleotidyltransferase ANT(4')-Ia  [1] (data from MRSA252)
    SA_RS00690immunoglobulin G-binding protein A  [1] (data from MRSA252)
    SA_RS01275formate acetyltransferase  [1] (data from MRSA252)
    SA_RS01310acetyl-CoA acetyltransferase  [1] (data from MRSA252)
    SA_RS0201530S ribosomal protein S6  [1] (data from MRSA252)
    SA_RS02095alkyl hydroperoxide reductase subunit C  [1] (data from MRSA252)
    SA_RS02145IMP dehydrogenase  [1] (data from MRSA252)
    SA_RS0265050S ribosomal protein L25/general stress protein Ctc  [1] (data from MRSA252)
    SA_RS02710cysteine synthase  [1] (data from MRSA252)
    SA_RS02810pyridoxal 5'-phosphate synthase lyase subunit PdxS  [1] (data from MRSA252)
    SA_RS0290550S ribosomal protein L11  [1] (data from MRSA252)
    SA_RS0291050S ribosomal protein L1  [1] (data from MRSA252)
    SA_RS0291550S ribosomal protein L10  [1] (data from MRSA252)
    SA_RS02930DNA-directed RNA polymerase subunit beta  [1] (data from MRSA252)
    SA_RS0295030S ribosomal protein S7  [1] (data from MRSA252)
    SA_RS02955elongation factor G  [1] (data from MRSA252)
    SA_RS02960elongation factor Tu  [1] (data from MRSA252)
    SA_RS03155phosphate acetyltransferase  [1] (data from MRSA252)
    SA_RS04020ribosomal subunit interface protein  [1] (data from MRSA252)
    SA_RS04140aldehyde dehydrogenase  [1] (data from MRSA252)
    SA_RS04145phosphoglycerate kinase  [1] (data from MRSA252)
    SA_RS04160enolase  [1] (data from MRSA252)
    SA_RS04575NADH dehydrogenase  [1] (data from MRSA252)
    SA_RS04680glucose-6-phosphate isomerase  [1] (data from MRSA252)
    SA_RS05295phosphocarrier protein HPr  [1] (data from MRSA252)
    SA_RS05350pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA_RS05355pyruvate dehydrogenase E1 component subunit beta  [1] (data from MRSA252)
    SA_RS05360dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex  [1] (data from MRSA252)
    SA_RS05365dihydrolipoyl dehydrogenase  [1] (data from MRSA252)
    SA_RS05860cell division protein FtsZ  [1] (data from MRSA252)
    SA_RS05895isoleucine--tRNA ligase  [1] (data from MRSA252)
    SA_RS0614050S ribosomal protein L19  [1] (data from MRSA252)
    SA_RS06165succinyl-CoA ligase subunit beta  [1] (data from MRSA252)
    SA_RS0622530S ribosomal protein S2  [1] (data from MRSA252)
    SA_RS06235elongation factor Ts  [1] (data from MRSA252)
    SA_RS0631530S ribosomal protein S15  [1] (data from MRSA252)
    SA_RS06690transketolase  [1] (data from MRSA252)
    SA_RS07060dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)
    SA_RS07205serine/threonine dehydratase  [1] (data from MRSA252)
    SA_RS07385DNA-binding protein HU  [1] (data from MRSA252)
    SA_RS07605phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)  [1] (data from MRSA252)
    SA_RS07880glycine--tRNA ligase  [1] (data from MRSA252)
    SA_RS07955molecular chaperone DnaK  [1] (data from MRSA252)
    SA_RS0829550S ribosomal protein L21  [1] (data from MRSA252)
    SA_RS08435trigger factor  [1] (data from MRSA252)
    SA_RS0846050S ribosomal protein L20  [1] (data from MRSA252)
    SA_RS08480threonine--tRNA ligase  [1] (data from MRSA252)
    SA_RS08505aldehyde dehydrogenase  [1] (data from MRSA252)
    SA_RS08545isocitrate dehydrogenase (NADP(+))  [1] (data from MRSA252)
    SA_RS08560pyruvate kinase  [1] (data from MRSA252)
    SA_RS08600universal stress protein  [1] (data from MRSA252)
    SA_RS08625universal stress protein UspA  [1] (data from MRSA252)
    SA_RS0867530S ribosomal protein S4  [1] (data from MRSA252)
    SA_RS08760formate--tetrahydrofolate ligase  [1] (data from MRSA252)
    SA_RS09810non-heme ferritin  [1] (data from MRSA252)
    SA_RS09850aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B  [1] (data from MRSA252)
    SA_RS10535molecular chaperone GroEL  [1] (data from MRSA252)
    SA_RS11145purine-nucleoside phosphorylase  [1] (data from MRSA252)
    SA_RS11430Asp23/Gls24 family envelope stress response protein  [1] (data from MRSA252)
    SA_RS1163050S ribosomal protein L17  [1] (data from MRSA252)
    SA_RS1164530S ribosomal protein S13  [1] (data from MRSA252)
    SA_RS1167050S ribosomal protein L15  [1] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [1] (data from MRSA252)
    SA_RS1169050S ribosomal protein L6  [1] (data from MRSA252)
    SA_RS1169530S ribosomal protein S8  [1] (data from MRSA252)
    SA_RS1170550S ribosomal protein L5  [1] (data from MRSA252)
    SA_RS1172030S ribosomal protein S17  [1] (data from MRSA252)
    SA_RS1173530S ribosomal protein S3  [1] (data from MRSA252)
    SA_RS1174050S ribosomal protein L22  [1] (data from MRSA252)
    SA_RS1174530S ribosomal protein S19  [1] (data from MRSA252)
    SA_RS1175050S ribosomal protein L2  [1] (data from MRSA252)
    SA_RS1175550S ribosomal protein L23  [1] (data from MRSA252)
    SA_RS1176050S ribosomal protein L4  [1] (data from MRSA252)
    SA_RS1176550S ribosomal protein L3  [1] (data from MRSA252)
    SA_RS13735malate:quinone oxidoreductase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 1.57 1.58 1.59 1.60 1.61 1.62 1.63 1.64 1.65 1.66 1.67 1.68 1.69 1.70 1.71 1.72 1.73 1.74 1.75 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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