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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08800 [old locus tag: SA1559 ]
  • pan locus tag?: SAUPAN004405000
  • symbol: SA_RS08800
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08800 [old locus tag: SA1559 ]
  • symbol: SA_RS08800
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1787508..1788866
  • length: 1359
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (1787508..1788866) NCBI
  • BioCyc: SA_RS08800 BioCyc
  • MicrobesOnline: see SA1559

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGACGCAGCAACAAAATGATAAAAGAACATTAAAAAATAAACACACTTATCAAAATGAA
    CCACTACCAAACCGTAAAGATTTTGTTGTTAGTTTTATAACTGGCGCGCTTGTTGGTTCA
    GCTTTAGGTTTGTATTTTAAAAATAAAGTTTATCAAAAAGCAGATGATTTAAAAGTCAAA
    GAACAAGAACTGTCGCAAAAGTTTGAAGAAAGAAAAACGCAACTTGAAGAAACGGTTGCC
    TATACAAAAGAACGTGTTGAAGGATTTTTAAACAAATCTAAAAATGAGCAAGCGGCATTA
    AAGGCACAACAAGCAGCGATAAAAGAAGAAGCAAGTGCAAATAATTTAAGTGATACATCA
    CAAGAAGCACAAGAGATTCAAGAAGCTAAAAGAGAAGCACAAGCAGAAGCGGATAAAAGT
    GTGGCTGTATCAAATGAAGAGTCAAAAGCATCGGCATTAAAAGTACAACAAGCAGCAATA
    AAAGAAGAAGCAAGTGCAAATAATTTAAGTGACACGTCACAAGAGGCACAAGAGATTCAA
    GAAGCTAAAAAAGAAGCACAAGCAGAAACAGATAAAAGTGCAGCTGTATCAAATGAAGAA
    CCAAAAGCAGTGGCATTGAAAGCACAACAAGCAGCGATAAAAGAAGAAGCAAGTGCAAAT
    AATTTAAGTGATACATCACAAGAGGCACAAGAGGTTCAAGAAGCTAAAAAAGAAGCACAA
    GCAGAGAAAGACAGTGACACATCAACTAAAGATGCAAGTGCAGCAAAGGTAGAAGTATCA
    AAACCAGAGTCACAAGCTGAAAGATTAGCAAACGCTGCAAAACAGAAGCAAGCTAAATTA
    ACACCAGGTTCAAAAGAGAGTCAATTAACTGAAGCGTTATTTGCAGAAAAACCAGTTGCT
    AAAAATGACTTGAAAGAAATTCCTCAATTAGTTACTAAAAAGAATGATGTATCAGAGACA
    GAGACGGTTAATATAGATAATAAAGACACTGTTAAACAAAAAGAAGCTAAATTTGAAAAT
    GGTGTTATTACACGTAAAGCTGATGAAAAAACAACTAATAATACAGCTGTTGACAAGAAA
    TCAGGTAAACAATCTAAAAAAACAACACCTTCAAATAAACGAAATGCATCAAAAGCATCT
    ACAAATAAAACTTCAGGTCAGAAAAAGCAACATAATAAGAAATCATCACAAGGTGCAAAG
    AAACAAAGTAGTTCAAGTAAGTTAACTCAAAAGAATAATCAAACTAGTAATAAGAATTCA
    AAAACAACAAATGCTAAATCATCCAATGCATCAAAAACGCCAAATGCTAAAGTTGAAAAA
    GCTAATAGTAAAATAGAGAAACGTACATTCAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1359

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS08800 [old locus tag: SA1559 ]
  • symbol: SA_RS08800
  • description: hypothetical protein
  • length: 452
  • theoretical pI: 10.0174
  • theoretical MW: 49513.2
  • GRAVY: -1.22721

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SA1559
  • PFAM:

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.11
    • Cellwall Score: 9.25
    • Extracellular Score: 0.63
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014086
    • TAT(Tat/SPI): 0.022843
    • LIPO(Sec/SPII): 0.00329
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTQQQNDKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGLYFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAYTKERVEGFLNKSKNEQAALKAQQAAIKEEASANNLSDTSQEAQEIQEAKREAQAEADKSVAVSNEESKASALKVQQAAIKEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEEPKAVALKAQQAAIKEEASANNLSDTSQEAQEVQEAKKEAQAEKDSDTSTKDASAAKVEVSKPESQAERLANAAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSETETVNIDNKDTVKQKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHNKKSSQGAKKQSSSSKLTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKANSKIEKRTFND

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA_RS00335PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA  [1] (data from MRSA252)
    SA_RS017105'-nucleotidase, lipoprotein e(P4) family  [1] (data from MRSA252)
    SA_RS0201530S ribosomal protein S6  [1] (data from MRSA252)
    SA_RS02700zinc metalloprotease  [1] (data from MRSA252)
    SA_RS0290550S ribosomal protein L11  [1] (data from MRSA252)
    SA_RS0291050S ribosomal protein L1  [1] (data from MRSA252)
    SA_RS0291550S ribosomal protein L10  [1] (data from MRSA252)
    SA_RS0292050S ribosomal protein L7/L12  [1] (data from MRSA252)
    SA_RS0294530S ribosomal protein S12  [1] (data from MRSA252)
    SA_RS03545hypothetical protein  [1] (data from MRSA252)
    SA_RS04160enolase  [1] (data from MRSA252)
    SA_RS05350pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA_RS05365dihydrolipoyl dehydrogenase  [1] (data from MRSA252)
    SA_RS05860cell division protein FtsZ  [1] (data from MRSA252)
    SA_RS05875cell division protein SepF  [1] (data from MRSA252)
    SA_RS0614050S ribosomal protein L19  [1] (data from MRSA252)
    SA_RS06755DNA topoisomerase 4 subunit A  [1] (data from MRSA252)
    SA_RS07060dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [1] (data from MRSA252)
    SA_RS07245cell cycle protein GpsB  [1] (data from MRSA252)
    SA_RS07385DNA-binding protein HU  [1] (data from MRSA252)
    SA_RS08030RNA-binding protein  [1] (data from MRSA252)
    SA_RS08245protein translocase subunit SecDF  [1] (data from MRSA252)
    SA_RS0829550S ribosomal protein L21  [1] (data from MRSA252)
    SA_RS08470translation initiation factor IF-3  [1] (data from MRSA252)
    SA_RS08600universal stress protein  [1] (data from MRSA252)
    SA_RS0867530S ribosomal protein S4  [1] (data from MRSA252)
    SA_RS08730serine protease  [1] (data from MRSA252)
    SA_RS08815DNA translocase FtsK  [1] (data from MRSA252)
    SA_RS10965ATP synthase subunit beta  [1] (data from MRSA252)
    SA_RS11015serine hydroxymethyltransferase  [1] (data from MRSA252)
    SA_RS1105050S ribosomal protein L31 type B  [1] (data from MRSA252)
    SA_RS1160030S ribosomal protein S9  [1] (data from MRSA252)
    SA_RS1163050S ribosomal protein L17  [1] (data from MRSA252)
    SA_RS1164030S ribosomal protein S11  [1] (data from MRSA252)
    SA_RS1167050S ribosomal protein L15  [1] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [1] (data from MRSA252)
    SA_RS1169050S ribosomal protein L6  [1] (data from MRSA252)
    SA_RS1170550S ribosomal protein L5  [1] (data from MRSA252)
    SA_RS1172030S ribosomal protein S17  [1] (data from MRSA252)
    SA_RS1173050S ribosomal protein L16  [1] (data from MRSA252)
    SA_RS1174050S ribosomal protein L22  [1] (data from MRSA252)
    SA_RS1174530S ribosomal protein S19  [1] (data from MRSA252)
    SA_RS1175050S ribosomal protein L2  [1] (data from MRSA252)
    SA_RS1175550S ribosomal protein L23  [1] (data from MRSA252)
    SA_RS1176050S ribosomal protein L4  [1] (data from MRSA252)
    SA_RS1176550S ribosomal protein L3  [1] (data from MRSA252)

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]