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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1998 [new locus tag: SA_RS11500 ]
  • pan locus tag?: SAUPAN005594000
  • symbol: lacR
  • pan gene symbol?: lacR
  • synonym:
  • product: lactose phosphotransferase system repressor

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA1998 [new locus tag: SA_RS11500 ]
  • symbol: lacR
  • product: lactose phosphotransferase system repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 2269393..2270148
  • length: 756
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    TTGAATAAACATGAACGTTTGGACGAAATTGCTAAACTAGTGAATAAAAAGGGCACGATA
    AGAACGAATGAAATCGTCGAAGGTTTAAATGTGTCTGATATGACAGTTCGAAGAGATTTA
    ATTGAGTTGGAAAATAAGGGCATTTTAACGAAGATTCATGGTGGTGCACGCAGTAATTCA
    ACATTTCAGTATAAAGAAATATCACATAAAGAAAAACATACTCGTCAAATTGCTGAAAAA
    AGATATATAGCTAGGAAAGCTGCATCATTAATTGAAGATGGGGATACTTTGTTTTTCGGA
    CCGGGAACAACAGTGGAACTATTAGCAGAAGAAGTCAATCATCATACGCTCACAATTATT
    ACGAATTGTTTGCCGGTGTATAAAATTTTGTTAGAAAAACAAACAGCACATTTTCGTGTC
    TATTTAATTGGTGGTGAAATGCGCCATATTACAGAAGCATTTGTAGGTGAAATGGCGAAT
    GCCATGTTGGAAAAACTAAGATTTAGCAAGATGTTCTTTAGTAGTAACGCAGTAAATAAA
    GGTGCTGTGATGACATCTACATTAGATGAAGCCTATACGCAACAATTGGCACTAAGTAAT
    TCAATTGAAAAATACTTGTTAATCGATCATACGAAAGTTGGCAAAGAAGATTTTACATCA
    TTTTGTCAGTTAAACGAATTGACTGCAGTGGTCATGGACTATGAAGATGAAGAAAAAGTA
    GAAACGATTAAAACATATATTGAAGTAGTCGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    756

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA1998 [new locus tag: SA_RS11500 ]
  • symbol: LacR
  • description: lactose phosphotransferase system repressor
  • length: 251
  • theoretical pI: 6.24703
  • theoretical MW: 28592.6
  • GRAVY: -0.312351

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 23.8)
    and 4 more
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 15.8)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 15.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 14.9)
    Signal transduction Regulatory functions DNA interactions iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 14.9)
  • TheSEED  :
    • Lactose phosphotransferase system repressor
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Lactose phosphotransferase system repressor
  • PFAM:
    ISOCOT_Fold (CL0246) DeoRC; DeoR C terminal sensor domain (PF00455; HMM-score: 162.4)
    and 8 more
    HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 81)
    HTH_11; HTH domain (PF08279; HMM-score: 27.6)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 21.3)
    FaeA; FaeA-like protein (PF04703; HMM-score: 17.7)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 16.4)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 14.8)
    Penicillinase_R; Penicillinase repressor (PF03965; HMM-score: 13.4)
    PaaX; PaaX-like protein (PF07848; HMM-score: 12.4)

Structure, modifications & cofactors[edit | edit source]

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.024817
    • TAT(Tat/SPI): 0.00181
    • LIPO(Sec/SPII): 0.000503
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRYIARKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]