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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS09820 [old locus tag: NWMN_1743 ]
  • pan locus tag?: SAUPAN004784000
  • symbol: NWMN_RS09820
  • pan gene symbol?: fumC
  • synonym:
  • product: class II fumarate hydratase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS09820 [old locus tag: NWMN_1743 ]
  • symbol: NWMN_RS09820
  • product: class II fumarate hydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 1944753..1946138
  • length: 1386
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1944753..1946138) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCAGTAAGAATTGAACATGATACTTTTGGAGAAATAGAAGTACCTGCAGATAAATAT
    TGGGGTGCTCAAACAGAAAGAAGTAAACGTAATTTCCCAGTTGGTAAAGAGCGTATGCCA
    ATCGAAGTAGTTTATGGTTTTGCACAACTAAAGCGTGCAGCAGCAATAGCTAATTTTGAT
    TTAGGAAAATTAAGCGAGGCAAAGAAAGATGCCATTGTATACGCATGTGATCAAATTTTA
    TCAGGTGAATTAGATGAACACTTCCCACTAGTTGTATGGCAAACAGGAAGCGGTACACAA
    AGTAATATGAATGTGAACGAAGTAGTAAGTTATGTTGCTAATATGTATTTAAAAGATCAT
    CAAAGTGATGAAAGTATCCACCCAAATGATGATGTAAATAAATCTCAAAGTTCGAATGAT
    ACATTCCCAACTGCTATGCACGTTGCATTATATCAAGAGGTTGAAACAAAATTAGAACCT
    GCATTAAAACTTTTAAGAAATACTTTGAAAGAAAAAGAAGATAAATTTGATTCAATTATT
    AAAATTGGTCGTACACATTTACAAGATGCAACGCCGATCAAACTAGGACAAGAGATTAGT
    GGCTGGCGTTATATGCTTGACCGTTGTGAAACAATGTTATCTGAATCTAAGAAGCACATT
    TTAAATCTTGCCATCGGTGGTACGGCTGTTGGTACTGGTATTAATGCGCATCCTGAATTT
    GGTGATAAAGTGGCACATTATATTTCAGAAAATACGGGTTATCCATTTGTATCTTCTGAA
    AATAAATTCCACGCACTTACAGCGCATGATGAAGTTGTTCAATTGCATGGAACATTGAAG
    GCATTAGCAGGAGACTTAATGAAAATTGCTAATGATGTGAGATGGTTGGCTTCAGGGCCA
    CGAGCTGGTTTGGCAGAAATTTCTATCCCTGAAAATGAACCAGGTTCATCAATTATGCCT
    GGTAAAGTTAATCCTACACAATGTGAAATGTTAACAATGGTTGCAGTCCAAGTAATGGGT
    AATGATACAGTTGTTGGCTTCGCAAGTTCACAAGGTAACTTTGAATTGAATGTTTATAAA
    CCAGTTATTATGCATAATACACTACAATCAATTTATCTTTTAGCTGATGGTATGGAAACA
    TTTAATAACAATTGTGCAGTGGGCATTGAACCAATCGAAGAGAATATTGATAATTATTTA
    AATCAATCATTAATGTTAGTTACTGCATTAAATCCACATATTGGTTATGAAAAAGCAGCT
    CAAATTGCTAAGAAAGCCCATAAAGAAGGTTTAACTTTAAAAGAATCTGCAATTCAAACT
    GGATATGTTACAGAAGAACAATTTGAAGCATGGATTAAACCAGAAGATATGGTAGATCCT
    CATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1386

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS09820 [old locus tag: NWMN_1743 ]
  • symbol: NWMN_RS09820
  • description: class II fumarate hydratase
  • length: 461
  • theoretical pI: 4.97681
  • theoretical MW: 51107.5
  • GRAVY: -0.329718

Function[edit source | edit]

  • reaction:
    EC 4.2.1.2?  ExPASy
    Fumarate hydratase(S)-malate = fumarate + H2O
  • TIGRFAM:
    MetabolismEnergy metabolismTCA cyclefumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 751.7)
    MetabolismEnergy metabolismAmino acids and aminesaspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 469.7)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisadenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 88.9)
    MetabolismEnergy metabolismOther3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 73)
    MetabolismAmino acid biosynthesisGlutamate familyargininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 60.3)
  • TheSEED:
  • PFAM:
    no clan definedLyase_1; Lyase (PF00206; HMM-score: 381)
    FumaraseC_C; Fumarase C C-terminus (PF10415; HMM-score: 91)
    DNA_primase_lrg (CL0242) DNA_primase_lrg; Eukaryotic and archaeal DNA primase, large subunit (PF04104; HMM-score: 16)
    KNTase_C (CL0291) NTase_sub_bind; Nucleotidyltransferase substrate binding protein like (PF08780; HMM-score: 15.4)
    no clan definedHsbA; Hydrophobic surface binding protein A (PF12296; HMM-score: 14.4)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.114
    • Ymax_pos: 56
    • Cmax: 0.119
    • Cmax_pos: 56
    • Smax: 0.169
    • Smax_pos: 53
    • Smean: 0.075
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446038369 NCBI
  • RefSeq: WP_000116224 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVVYGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSNMNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEPALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKKHILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLHGTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMVAVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPIEENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEAWIKPEDMVDPH

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS09820 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23

Relevant publications[edit source | edit]