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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1743 [new locus tag: NWMN_RS09820 ]
  • pan locus tag?: SAUPAN004784000
  • symbol: fumC
  • pan gene symbol?: fumC
  • synonym:
  • product: fumarate hydratase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1743 [new locus tag: NWMN_RS09820 ]
  • symbol: fumC
  • product: fumarate hydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 1944753..1946138
  • length: 1386
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCAGTAAGAATTGAACATGATACTTTTGGAGAAATAGAAGTACCTGCAGATAAATAT
    TGGGGTGCTCAAACAGAAAGAAGTAAACGTAATTTCCCAGTTGGTAAAGAGCGTATGCCA
    ATCGAAGTAGTTTATGGTTTTGCACAACTAAAGCGTGCAGCAGCAATAGCTAATTTTGAT
    TTAGGAAAATTAAGCGAGGCAAAGAAAGATGCCATTGTATACGCATGTGATCAAATTTTA
    TCAGGTGAATTAGATGAACACTTCCCACTAGTTGTATGGCAAACAGGAAGCGGTACACAA
    AGTAATATGAATGTGAACGAAGTAGTAAGTTATGTTGCTAATATGTATTTAAAAGATCAT
    CAAAGTGATGAAAGTATCCACCCAAATGATGATGTAAATAAATCTCAAAGTTCGAATGAT
    ACATTCCCAACTGCTATGCACGTTGCATTATATCAAGAGGTTGAAACAAAATTAGAACCT
    GCATTAAAACTTTTAAGAAATACTTTGAAAGAAAAAGAAGATAAATTTGATTCAATTATT
    AAAATTGGTCGTACACATTTACAAGATGCAACGCCGATCAAACTAGGACAAGAGATTAGT
    GGCTGGCGTTATATGCTTGACCGTTGTGAAACAATGTTATCTGAATCTAAGAAGCACATT
    TTAAATCTTGCCATCGGTGGTACGGCTGTTGGTACTGGTATTAATGCGCATCCTGAATTT
    GGTGATAAAGTGGCACATTATATTTCAGAAAATACGGGTTATCCATTTGTATCTTCTGAA
    AATAAATTCCACGCACTTACAGCGCATGATGAAGTTGTTCAATTGCATGGAACATTGAAG
    GCATTAGCAGGAGACTTAATGAAAATTGCTAATGATGTGAGATGGTTGGCTTCAGGGCCA
    CGAGCTGGTTTGGCAGAAATTTCTATCCCTGAAAATGAACCAGGTTCATCAATTATGCCT
    GGTAAAGTTAATCCTACACAATGTGAAATGTTAACAATGGTTGCAGTCCAAGTAATGGGT
    AATGATACAGTTGTTGGCTTCGCAAGTTCACAAGGTAACTTTGAATTGAATGTTTATAAA
    CCAGTTATTATGCATAATACACTACAATCAATTTATCTTTTAGCTGATGGTATGGAAACA
    TTTAATAACAATTGTGCAGTGGGCATTGAACCAATCGAAGAGAATATTGATAATTATTTA
    AATCAATCATTAATGTTAGTTACTGCATTAAATCCACATATTGGTTATGAAAAAGCAGCT
    CAAATTGCTAAGAAAGCCCATAAAGAAGGTTTAACTTTAAAAGAATCTGCAATTCAAACT
    GGATATGTTACAGAAGAACAATTTGAAGCATGGATTAAACCAGAAGATATGGTAGATCCT
    CATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1386

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1743 [new locus tag: NWMN_RS09820 ]
  • symbol: FumC
  • description: fumarate hydratase
  • length: 461
  • theoretical pI: 4.97681
  • theoretical MW: 51107.5
  • GRAVY: -0.329718

Function[edit source | edit]

  • reaction:
    EC 4.2.1.2?  ExPASy
    Fumarate hydratase(S)-malate = fumarate + H2O
  • TIGRFAM:
    MetabolismEnergy metabolismTCA cyclefumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 751.7)
    MetabolismEnergy metabolismAmino acids and aminesaspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 469.7)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisadenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 88.9)
    MetabolismEnergy metabolismOther3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 73)
    MetabolismAmino acid biosynthesisGlutamate familyargininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 60.3)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismTCA Cycle Fumarate hydratase class II (EC 4.2.1.2) 
  • PFAM:
    no clan definedLyase_1; Lyase (PF00206; HMM-score: 381)
    FumaraseC_C; Fumarase C C-terminus (PF10415; HMM-score: 91)
    DNA_primase_lrg (CL0242) DNA_primase_lrg; Eukaryotic and archaeal DNA primase, large subunit (PF04104; HMM-score: 16)
    KNTase_C (CL0291) NTase_sub_bind; Nucleotidyltransferase substrate binding protein like (PF08780; HMM-score: 15.4)
    no clan definedHsbA; Hydrophobic surface binding protein A (PF12296; HMM-score: 14.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_2131(adk)adenylate kinase  [1] (data from MRSA252)
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_1483(dnaK)molecular chaperone DnaK  [1] (data from MRSA252)
    NWMN_0551(eutD)phosphotransacetylase  [1] (data from MRSA252)
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_0741(gapA)glyceraldehyde 3-phosphate dehydrogenase 1  [1] (data from MRSA252)
    NWMN_1838(gatA)aspartyl/glutamyl-tRNA amidotransferase subunit A  [1] (data from MRSA252)
    NWMN_0828(gudB)NAD-specific glutamate dehydrogenase  [1] (data from MRSA252)
    NWMN_0833(pgi)glucose-6-phosphate isomerase  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_2133(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_2125(rplQ)50S ribosomal protein L17  [1] (data from MRSA252)
    NWMN_2150(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    NWMN_0504(rpoB)DNA-directed RNA polymerase subunit beta  [1] (data from MRSA252)
    NWMN_1166(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_2016(upp)uracil phosphoribosyltransferase  [1] (data from MRSA252)
    NWMN_0346acetyl-CoA acetyltransferase  [1] (data from MRSA252)
    NWMN_0632hypothetical protein  [1] (data from MRSA252)
    NWMN_0638hypothetical protein  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)
    NWMN_0811hypothetical protein  [1] (data from MRSA252)
    NWMN_0885hypothetical protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.114
    • Ymax_pos: 56
    • Cmax: 0.119
    • Cmax_pos: 56
    • Smax: 0.169
    • Smax_pos: 53
    • Smean: 0.075
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVVYGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSNMNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEPALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKKHILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLHGTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMVAVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPIEENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEAWIKPEDMVDPH

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]