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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0557 [new locus tag: SA_RS03220 ]
  • pan locus tag?: SAUPAN002463000
  • symbol: SA0557
  • pan gene symbol?: iolS
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0557 [new locus tag: SA_RS03220 ]
  • symbol: SA0557
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 650610..651548
  • length: 939
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAGCAATTTGTAAACTTAGGTAAATCTGATGTTGAAGTGTTTCCAATCGCACTTGGG
    ACGAACGCAGTAGGTGGGCATAATTTATATCCGAACTTAGATGAAGAACAAGGAAAAGAT
    GTTGTTCGTCAAGCCATTAATCATGGTATTAATTTATTAGATACGGCATATATTTATGGG
    CCAGAACGATCAGAAGAATTGGTTGGAGAAGTTGTTAAGGAATATCCGCGAGAGCAAATT
    AAAATTGCTACGAAAGGGTCTCATGAATTTGATGAAAATCAAGAAGTACATCAGAACAAT
    CAACCGGAATATTTAAAACAACAAGTTGAGAATAGTTTGAAACGTCTACAAACTGATTAT
    ATCGATTTATATTATATTCATTTTCCTGATGACAACACTCCGAAAGATCAAGCAGTTGCA
    GCATTACAAGAGCTTAAGGAACAAGGGAAGATTAAAGCCATTGGTGTATCAAATTTCACA
    TTAGATCAACTTAAAGAAGCAAATAAAGATGGTTACGTTGATGTTGTACAGTTAGAATAT
    AATTTATTGCACCGCGAAAATGAGGCAGTATTGCAATATTGTGTTGATCACCTAATCACA
    TTTATTCCTTATTTCCCATTAGCATCCGGTATTTTAGCTGGAAAATATGATGAGAACACT
    AAATTTAGTGACCATCGTACTACACGTCGTGATTTTATACCTGGTGTTTTTGAAGAGAAT
    GTGCGTCGCGTAAAAGCTTTGGAAAGCCTAGCTGCAGCACATCAAACTTCAATTGCGAAC
    ATTGTATTAGCATTTTATTTAACGAGACCAGCTATCGATGTGATTATTCCTGGTGCAAAA
    CGTGCAGAACAAGTCATTGAAAATATTAAAGCTGCAGATATCGTTTTATCAGATGATGAG
    ATTCAATATATCGATGAATTGTTTCCAATTGAAGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0557 [new locus tag: SA_RS03220 ]
  • symbol: SA0557
  • description: hypothetical protein
  • length: 312
  • theoretical pI: 4.54075
  • theoretical MW: 35444.5
  • GRAVY: -0.416346

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    voltage-dependent potassium channel beta subunit (TIGR01293; HMM-score: 87.3)
  • TheSEED:  
    myo-inositol operon protein IolS 
  • PFAM:
    no clan definedAldo_ket_red; Aldo/keto reductase family (PF00248; HMM-score: 251.2)
    NADP_Rossmann (CL0063) Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 14.1)
    no clan definedRasGAP_C; RasGAP C-terminus (PF03836; HMM-score: 13.2)
    HAD (CL0137) Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 12.9)
    Glyco_hydro_tim (CL0058) Glyco_hydro_1; Glycosyl hydrolase family 1 (PF00232; HMM-score: 11.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA1150(glnA)glutamine-ammonia ligase  [1] (data from MRSA252)
    SA1342(gnd)6-phosphogluconate dehydrogenase  [1] (data from MRSA252)
    SA1112(infB)translation initiation factor IF-2  [1] (data from MRSA252)
    SA0232(lctE)L-lactate dehydrogenase  [1] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA2046(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    SA2035(rplE)50S ribosomal protein L5  [1] (data from MRSA252)
    SA0497(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SA2022(rplQ)50S ribosomal protein L17  [1] (data from MRSA252)
    SA2045(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    SA1471(rpmA)50S ribosomal protein L27  [1] (data from MRSA252)
    SA1503(rpmI)50S ribosomal protein L35  [1] (data from MRSA252)
    SA0501(rpoC)DNA-directed RNA polymerase subunit beta'  [1] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SA2041(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SA0352(rpsF)30S ribosomal protein S6  [1] (data from MRSA252)
    SA0354(rpsR)30S ribosomal protein S18  [1] (data from MRSA252)
    SA1382(sodA)superoxide dismutase SodA  [1] (data from MRSA252)
    SA0107(spa)immunoglobulin G binding protein A  [1] (data from MRSA252)
    SA0992(trxA)thioredoxin  [1] (data from MRSA252)
    SA1100(tsf)elongation factor Ts  [1] (data from MRSA252)
    SA1914(upp)uracil phosphoribosyltransferase  [1] (data from MRSA252)
    SA1532hypothetical protein  [1] (data from MRSA252)
    SA2399fructose-1,6-bisphosphate aldolase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.152
    • Ymax_pos: 27
    • Cmax: 0.11
    • Cmax_pos: 27
    • Smax: 0.263
    • Smax_pos: 22
    • Smean: 0.194
    • D: 0.169
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQAINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQPEYLKQQVENSLKRLQTDYIDLYYIHFPDDNTPKDQAVAALQELKEQGKIKAIGVSNFTLDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHLITFIPYFPLASGILAGKYDENTKFSDHRTTRRDFIPGVFEENVRRVKALESLAAAHQTSIANIVLAFYLTRPAIDVIIPGAKRAEQVIENIKAADIVLSDDEIQYIDELFPIED

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24

Relevant publications[edit source | edit]