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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08940 [old locus tag: SACOL1747 ]
  • pan locus tag?: SAUPAN004347000
  • symbol: SACOL_RS08940
  • pan gene symbol?: accA
  • synonym:
  • product: acetyl-CoA carboxylase carboxyl transferase subunit alpha

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS08940 [old locus tag: SACOL1747 ]
  • symbol: SACOL_RS08940
  • product: acetyl-CoA carboxylase carboxyl transferase subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 1784942..1785886
  • length: 945
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1784942..1785886) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTTAGATTTTGAAAAACCACTTTTTGAAATTCGAAATAAAATTGAATCTTTAAAAGAA
    TCTCAAGATAAAAATGATGTGGATTTACAAGAAGAAATTGACATGCTTGAAGCGTCATTG
    GAACGAGAAACTAAAAAAATATATACAAATCTAAAACCATGGGATCGTGTGCAAATTGCG
    CGTTTGCAAGAAAGACCTACGACCCTAGATTATATTCCATATATCTTTGATTCGTTTATG
    GAACTACATGGTGATCGTAATTTTAGAGATGATCCAGCAATGATTGGTGGTATTGGCTTT
    TTAAATGGTCGTGCTGTTACAGTTATTGGACAACAACGTGGAAAAGATACAAAAGATAAT
    ATTTATCGAAATTTTGGTATGGCGCATCCAGAAGGTTATCGAAAAGCATTACGTTTAATG
    AAACAAGCTGAAAAATTCAATCGTCCTATCTTTACATTTATAGATACAAAAGGTGCATAT
    CCTGGTAAAGCTGCTGAAGAACGTGGACAAAGTGAATCTATCGCAACAAATTTGATTGAG
    ATGGCTTCATTAAAAGTACCAGTTATTGCGATTGTCATTGGTGAAGGTGGCAGTGGAGGT
    GCTCTAGGTATTGGTATTGCCAATAAAGTATTGATGTTAGAGAATAGTACTTACTCTGTT
    ATATCTCCTGAAGGTGCAGCGGCATTATTATGGAAAGACAGTAATTTGGCTAAAATTGCA
    GCTGAAACAATGAAAATTACTGCCCATGATATTAAGCAATTAGGTATTATAGATGATGTC
    ATTTCTGAACCACTTGGCGGTGCACATAAAGATATTGAACAGCAAGCTTTAGCTATTAAA
    TCAGCGTTTGTTGCACAGTTAGATTCACTTGAGTCATTATCACGTGATGAAATTGCTAAT
    GATCGCTTTGAAAAATTCAGAAATATCGGTTCTTATATAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
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    840
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    945

Protein[edit source | edit]

Protein Data Bank: 2F9I
Protein Data Bank: 5KDR

General[edit source | edit]

  • locus tag: SACOL_RS08940 [old locus tag: SACOL1747 ]
  • symbol: SACOL_RS08940
  • description: acetyl-CoA carboxylase carboxyl transferase subunit alpha
  • length: 314
  • theoretical pI: 4.95687
  • theoretical MW: 35069.7
  • GRAVY: -0.340764

Function[edit source | edit]

  • reaction:
    EC 6.4.1.2?  ExPASy
    Acetyl-CoA carboxylaseATP + acetyl-CoA + HCO3(-) = ADP + phosphate + malonyl-CoA
  • TIGRFAM:
    MetabolismFatty acid and phospholipid metabolismBiosynthesisacetyl-CoA carboxylase, carboxyl transferase, alpha subunit (TIGR00513; EC 6.4.1.2; HMM-score: 486.1)
    biotin-independent malonate decarboxylase, gamma subunit (TIGR03134; EC 4.1.1.88; HMM-score: 45.6)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmethylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 29.6)
    MetabolismEnergy metabolismFermentationmethylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 29.6)
    MetabolismEnergy metabolismATP-proton motive force interconversionmethylmalonyl-CoA decarboxylase alpha subunit (TIGR01117; EC 4.1.1.41; HMM-score: 29.6)
  • TheSEED:
  • PFAM:
    ClpP_crotonase (CL0127) ACCA; Acetyl co-enzyme A carboxylase carboxyltransferase alpha subunit (PF03255; HMM-score: 211.4)
    Carboxyl_trans; Carboxyl transferase domain (PF01039; HMM-score: 55.1)
    MdcE; Malonate decarboxylase gamma subunit (MdcE) (PF06833; HMM-score: 18.7)
    ECH_1; Enoyl-CoA hydratase/isomerase (PF00378; HMM-score: 17)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 11
    • Cmax: 0.118
    • Cmax_pos: 30
    • Smax: 0.138
    • Smax_pos: 3
    • Smean: 0.111
    • D: 0.108
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446806389 NCBI
  • RefSeq: WP_000883645 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERETKKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLNGRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAYPGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTYSVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQALAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS08940 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]