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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02341
  • pan locus tag?: SAUPAN005394000
  • symbol: SAOUHSC_02341
  • pan gene symbol?: atpD
  • synonym:
  • product: F0F1 ATP synthase subunit beta

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02341
  • symbol: SAOUHSC_02341
  • product: F0F1 ATP synthase subunit beta
  • replicon: chromosome
  • strand: -
  • coordinates: 2167863..2169275
  • length: 1413
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGAATTGGCCGTGTAACTCAAGTTATGGGTCCTGTAATTGATGTTCGATTTGAACAT
    AACGAAGTTCCTAAAATTAATAACGCCTTGGTTATTGATGTGCCTAAAGAAGAAGGTACA
    ATACAACTAACATTAGAAGTTGCGCTGCAATTAGGTGACGACGTTGTTCGTACAATTGCG
    ATGGATTCAACTGATGGTGTCCAAAGAGGCATGGATGTAAAAGATACAGGCAAAGAAATT
    AGTGTACCTGTTGGTGACGAAACATTAGGTCGTGTATTTAATGTACTAGGTGAAACAATT
    GACCTTAAAGAAGAAATTAGTGATTCTGTTCGCCGCGATCCTATCCATCGTCAAGCACCA
    GCATTCGATGAACTTTCAACAGAAGTTCAAATTTTAGAAACAGGTATTAAAGTAGTAGAT
    TTACTAGCACCTTATATTAAAGGTGGTAAAATCGGATTGTTCGGTGGTGCCGGTGTAGGT
    AAAACAGTATTAATCCAAGAATTAATTAACAACATCGCTCAAGAGCACGGTGGTATTTCT
    GTATTCGCCGGTGTAGGTGAACGTACTCGTGAAGGTAACGATTTATACTTCGAAATGAGT
    GACAGTGGTGTAATTAAGAAAACAGCCATGGTATTCGGGCAAATGAATGAGCCACCTGGT
    GCACGTATGCGTGTTGCATTATCTGGTTTAACAATGGCTGAATATTTCCGTGACGAACAA
    GGTCAAGACGTATTATTATTCATCGATAACATTTTCAGATTTACACAAGCTGGTTCTGAG
    GTATCTGCATTATTAGGTCGTATGCCTTCTGCAGTAGGTTACCAACCAACACTTGCTACT
    GAAATGGGACAATTACAAGAACGTATTACGTCTACAACAAAAGGATCAGTTACTTCTATT
    CAAGCGGTATTCGTACCTGCCGATGACTATACTGACCCAGCGCCTGCGACAGCGTTTGCC
    CATTTAGATGCAACTACAAACTTAGAACGTAAATTAACTGAAATGGGTATTTATCCAGCC
    GTGGATCCATTAGCGTCTACATCAAGAGCATTGGAACCATCAATTGTAGGTCAAGAACAT
    TATGAAGTAGCTCGTGATGTACAATCAACACTTCAAAAATACCGTGAATTACAAGATATC
    ATTGCTATCTTAGGTATGGACGAATTATCTGATGAAGATAAACAAACAGTTGAACGCGCA
    CGTAGAATTCAATTCTTCTTATCTCAAAACTTCCACGTAGCGGAACAATTTACTGGTCAA
    AAAGGTTCTTATGTACCTGTTAAGACAACAGTTGCAAACTTTAAAGATATCTTAGATGGT
    AAATATGACCATATTCCAGAAGATGCATTCCGTTTAGTTGGTAGCATGGATGATGTTATT
    GCAAAAGCTAAAGATATGGGTGTTGAAGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1413

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02341
  • symbol: SAOUHSC_02341
  • description: F0F1 ATP synthase subunit beta
  • length: 470
  • theoretical pI: 4.42228
  • theoretical MW: 51399.9
  • GRAVY: -0.161915

Function[edit source | edit]

  • reaction:
    EC 3.6.3.14?  ExPASy
    H+-transporting two-sector ATPaseATP + H2O + H+(In) = ADP + phosphate + H+(Out)
  • TIGRFAM:
    MetabolismEnergy metabolismATP-proton motive force interconversionATP synthase F1, beta subunit (TIGR01039; EC 3.6.3.14; HMM-score: 844.9)
    and 16 more
    alternate F1F0 ATPase, F1 subunit beta (TIGR03305; EC 3.6.3.-; HMM-score: 586.7)
    MetabolismEnergy metabolismATP-proton motive force interconversionATPase, FliI/YscN family (TIGR01026; EC 3.6.3.14; HMM-score: 234.1)
    Cellular processesCellular processesPathogenesistype III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 233.1)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 233.1)
    Cellular processesCellular processesChemotaxis and motilityflagellar protein export ATPase FliI (TIGR03496; EC 3.6.3.14; HMM-score: 229.2)
    flagellar protein export ATPase FliI (TIGR03498; EC 3.6.3.14; HMM-score: 226.1)
    Cellular processesCellular processesChemotaxis and motilityflagellar protein export ATPase FliI (TIGR03497; EC 3.6.3.14; HMM-score: 216)
    MetabolismEnergy metabolismATP-proton motive force interconversionATP synthase archaeal, B subunit (TIGR01041; EC 3.6.3.14; HMM-score: 143.4)
    MetabolismEnergy metabolismATP-proton motive force interconversionATP synthase archaeal, A subunit (TIGR01043; EC 3.6.3.14; HMM-score: 134.6)
    alternate F1F0 ATPase, F1 subunit alpha (TIGR03324; EC 3.6.3.-; HMM-score: 133.2)
    MetabolismEnergy metabolismATP-proton motive force interconversionATP synthase F1, alpha subunit (TIGR00962; EC 3.6.3.14; HMM-score: 132.4)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsV-type ATPase, A subunit (TIGR01042; EC 3.6.3.14; HMM-score: 121.4)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsV-type ATPase, B subunit (TIGR01040; EC 3.6.3.14; HMM-score: 103)
    Genetic information processingTranscriptionTranscription factorstranscription termination factor Rho (TIGR00767; EC 3.6.4.-; HMM-score: 41.1)
    Genetic information processingProtein synthesisTranslation factorsribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 13)
    Genetic information processingProtein synthesistRNA and rRNA base modificationpseudouridine synthase Rlu family protein, TIGR01621 (TIGR01621; EC 5.4.99.-; HMM-score: 11.9)
  • TheSEED:  
    RespirationATP synthasesF0F1-type ATP synthase ATP synthase beta chain (EC 3.6.3.14) 
  • PFAM:
    P-loop_NTPase (CL0023) ATP-synt_ab; ATP synthase alpha/beta family, nucleotide-binding domain (PF00006; HMM-score: 226.3)
    and 12 more
    HAS-barrel (CL0275) ATP-synt_ab_N; ATP synthase alpha/beta family, beta-barrel domain (PF02874; HMM-score: 79.1)
    P-loop_NTPase (CL0023) RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 23.7)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 19.3)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 16.9)
    ABC_tran; ABC transporter (PF00005; HMM-score: 15.8)
    NACHT; NACHT domain (PF05729; HMM-score: 14.9)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 14.1)
    AAA_19; AAA domain (PF13245; HMM-score: 14)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 13.6)
    ATPase; KaiC (PF06745; HMM-score: 11.9)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 10.9)
    TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 10.9)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.12
    • Ymax_pos: 26
    • Cmax: 0.114
    • Cmax_pos: 63
    • Smax: 0.17
    • Smax_pos: 17
    • Smean: 0.106
    • D: 0.115
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLTLEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDLKEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVGKTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEPPGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTEMGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDEDKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAFRLVGSMDDVIAKAKDMGVEV

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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