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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1644 [new locus tag: SAUSA300_RS08975 ]
  • pan locus tag?: SAUPAN004324000
  • symbol: pyk
  • pan gene symbol?: pykA
  • synonym:
  • product: pyruvate kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_1644 [new locus tag: SAUSA300_RS08975 ]
  • symbol: pyk
  • product: pyruvate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1804763..1806520
  • length: 1758
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAGAAAAACTAAAATTGTATGTACAATTGGACCAGCTTCAGAATCAGAAGAAATGATT
    GAGAAATTAATCAATGCTGGTATGAACGTTGCACGATTAAACTTTTCACATGGTAGTCAT
    GAAGAGCATAAAGGTAGAATTGATACAATTCGTAAAGTAGCTAAAAGATTAGACAAAATT
    GTAGCAATTTTATTAGATACAAAAGGTCCAGAAATTCGTACGCATAATATGAAAGACGGT
    ATCATTGAACTTGAACGTGGCAACGAAGTTATTGTTAGCATGAATGAAGTTGAAGGAACA
    CCTGAAAAGTTCTCAGTAACATATGAAAACTTAATTAACGATGTTCAAGTAGGTTCATAC
    ATTTTACTTGATGATGGCTTAATTGAATTACAAGTTAAAGATATTGACCATGCTAAAAAA
    GAAGTTAAATGTGATATTTTAAACTCTGGTGAGCTTAAAAACAAAAAAGGTGTTAACTTA
    CCTGGCGTAAGAGTAAGTTTACCTGGTATTACAGAAAAAGATGCTGAAGATATCCGTTTC
    GGTATTAAAGAAAATGTTGACTTCATTGCAGCAAGTTTCGTACGTCGTCCTAGTGATGTT
    TTAGAAATTCGTGAAATTTTAGAAGAACAAAAAGCTAACATTTCAGTATTCCCTAAAATT
    GAAAACCAAGAAGGTATTGATAATATTGCGGAAATTCTTGAAGTGTCTGATGGTTTAATG
    GTTGCACGTGGTGACATGGGTGTTGAAATTCCACCTGAAAAAGTACCAATGGTTCAAAAA
    GATTTAATCAGACAATGTAACAAATTAGGTAAACCAGTTATTACAGCTACACAAATGTTA
    GATTCTATGCAACGTAACCCACGTGCTACACGTGCAGAAGCTAGTGACGTTGCCAACGCA
    ATCTATGATGGTACAGATGCAGTAATGTTATCTGGTGAAACTGCTGCTGGTTTATATCCT
    GAAGAAGCTGTTAAAACAATGAGAAATATTGCTGTATCAGCTGAAGCAGCCCAAGATTAC
    AAAAAGTTATTGTCAGATCGTACTAAATTAGTTGAAACTTCATTAGTGAATGCTATCGGT
    ATTTCGGTTGCACATACAGCTTTAAACTTAAATGTTAAAGCAATTGTAGCTGCTACTGAA
    AGTGGTTCAACGGCACGTACTATCTCCAAATATCGTCCACATTCAGACATTATTGCGGTG
    ACTCCAAGTGAAGAAACTGCACGTCAATGTTCAATTGTTTGGGGAGTTCAACCTGTAGTT
    AAAAAAGGACGTAAGAGTACAGATGCATTGTTAAACAATGCAGTTGCAACAGCTGTTGAA
    ACTGGTAGAGTATCTAATGGTGATTTAATCATTATTACTGCTGGTGTACCAACTGGTGAA
    ACTGGAACTACTAATATGATGAAAATCCACCTAGTTGGTGACGAAATTGCTAATGGTCAA
    GGTATTGGACGTGGATCAGTTGTTGGTACTACGTTAGTTGCTGAAACTGTTAAAGATTTA
    GAAGGTAAAGATTTATCTGACAAAGTTATCGTTACTAACTCAATCGATGAAACGTTTGTA
    CCTTATGTAGAAAAAGCTTTAGGCTTAATTACAGAAGAAAATGGTATTACATCACCAAGT
    GCAATTGTTGGTTTAGAAAAAGGTATTCCAACAGTTGTAGGTGTAGAAAAAGCTGTTAAA
    AACATAAGCAATAACATGTTAGTTACGATTGATGCTGCTCAAGGTAAAATCTTTGAAGGA
    TATGCAAACGTACTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1758

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_1644 [new locus tag: SAUSA300_RS08975 ]
  • symbol: Pyk
  • description: pyruvate kinase
  • length: 585
  • theoretical pI: 4.99555
  • theoretical MW: 63101.9
  • GRAVY: -0.127863

Function[edit source | edit]

  • reaction:
    EC 2.7.1.40?  ExPASy
    Pyruvate kinaseATP + pyruvate = ADP + phosphoenolpyruvate
  • TIGRFAM:
    MetabolismEnergy metabolismGlycolysis/gluconeogenesispyruvate kinase (TIGR01064; EC 2.7.1.40; HMM-score: 619.1)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesisphosphoenolpyruvate synthase (TIGR01418; EC 2.7.9.2; HMM-score: 56.3)
    phosphoenolpyruvate-protein phosphotransferase (TIGR01417; EC 2.7.3.9; HMM-score: 27.2)
    2-dehydro-3-deoxyglucarate aldolase (TIGR03239; EC 4.1.2.20; HMM-score: 15.6)
    2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (TIGR02311; EC 4.1.2.-; HMM-score: 14.4)
  • TheSEED:  
    Fermentations: Mixed acid Pyruvate kinase (EC 2.7.1.40) / Phosphohistidine swiveling domain 
    Glycerate metabolism Pyruvate kinase (EC 2.7.1.40) / Phosphohistidine swiveling domain 
    Glycolysis and Gluconeogenesis Pyruvate kinase (EC 2.7.1.40) / Phosphohistidine swiveling domain 
    Pyruvate metabolism I: anaplerotic reactions, PEP Pyruvate kinase (EC 2.7.1.40) / Phosphohistidine swiveling domain 
  • PFAM:
    PK_TIM (CL0151) PK; Pyruvate kinase, barrel domain (PF00224; HMM-score: 511.6)
    no clan definedPK_C; Pyruvate kinase, alpha/beta domain (PF02887; HMM-score: 127.9)
    Leu-IlvD (CL0364) PEP-utilizers; PEP-utilising enzyme, mobile domain (PF00391; HMM-score: 55.7)
    HTH (CL0123) IF2_N; Translation initiation factor IF-2, N-terminal region (PF04760; HMM-score: 19)
    PK_TIM (CL0151) HpcH_HpaI; HpcH/HpaI aldolase/citrate lyase family (PF03328; HMM-score: 14.7)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.136
    • Ymax_pos: 23
    • Cmax: 0.134
    • Cmax_pos: 42
    • Smax: 0.256
    • Smax_pos: 1
    • Smean: 0.167
    • D: 0.148
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHKGRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPEKFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNLPGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFPKIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITATQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSAEAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYRPHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLIIITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDKVIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNMLVTIDAAQGKIFEGYANVL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 1.57 1.58 1.59 1.60 1.61 1.62 1.63 1.64 1.65 1.66 1.67 1.68 1.69 1.70 1.71 1.72 1.73 1.74 1.75 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]