From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1370 [new locus tag: SACOL_RS06975 ]
  • pan locus tag?: SAUPAN003709000
  • symbol: rpsN
  • pan gene symbol?: rpsN2
  • synonym:
  • product: 30S ribosomal protein S14

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1370 [new locus tag: SACOL_RS06975 ]
  • symbol: rpsN
  • product: 30S ribosomal protein S14
  • replicon: chromosome
  • strand: +
  • coordinates: 1376011..1376280
  • length: 270
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    ATGGCTAAGAAATCTAAAATAGCAAAAGAGAGAAAAAGAGAAGAGTTAGTAAATAAATAT
    TACGAATTACGTAAAGAGTTAAAAGCAAAAGGTGATTACGAAGCGTTAAGAAAATTACCA
    AGAGATTCATCACCTACACGTTTAACTAGAAGATGTAAAGTAACTGGAAGACCTAGAGGT
    GTATTACGTAAATTTGAAATGTCTCGTATTGCGTTTAGAGAACATGCGCACAAAGGACAA
    ATTCCAGGTGTTAAAAAATCAAGTTGGTAA
    60
    120
    180
    240
    270

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1370 [new locus tag: SACOL_RS06975 ]
  • symbol: RpsN
  • description: 30S ribosomal protein S14
  • length: 89
  • theoretical pI: 11.4256
  • theoretical MW: 10540.3
  • GRAVY: -1.18989

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • SSU ribosomal protein S14p (S29e)
    • SSU ribosomal protein S14p (S29e), zinc-independent
    Protein Metabolism Protein biosynthesis Ribosome SSU bacterial  SSU ribosomal protein S14p (S29e)
  • PFAM:
    no clan defined Ribosomal_S14; Ribosomal protein S14p/S29e (PF00253; HMM-score: 97.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013347
    • TAT(Tat/SPI): 0.00948
    • LIPO(Sec/SPII): 0.00334
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSSPTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SACOL1760(ackA)acetate kinase  [2] (data from MRSA252)
    SACOL1385(acnA)aconitate hydratase  [2] (data from MRSA252)
    SACOL2656(arcB2)ornithine carbamoyltransferase  [2] (data from MRSA252)
    SACOL0842(eno)phosphopyruvate hydratase  [2] (data from MRSA252)
    SACOL0634(eutD)phosphotransacetylase  [2] (data from MRSA252)
    SACOL1245(fabG1)3-oxoacyl-ACP reductase  [2] (data from MRSA252)
    SACOL2622(fdaB)fructose-1,6-bisphosphate aldolase  [2] (data from MRSA252)
    SACOL1329(femC)glutamine synthetase  [2] (data from MRSA252)
    SACOL1782(fhs)formate--tetrahydrofolate ligase  [2] (data from MRSA252)
    SACOL1198(ftsA)cell division protein FtsA  [2] (data from MRSA252)
    SACOL0593(fusA)elongation factor G  [2] (data from MRSA252)
    SACOL0838(gapA1)glyceraldehyde 3-phosphate dehydrogenase  [2] (data from MRSA252)
    SACOL0877(gcvH)glycine cleavage system protein H  [2] (data from MRSA252)
    SACOL2145(glmS)glucosamine--fructose-6-phosphate aminotransferase  [2] (data from MRSA252)
    SACOL2016(groEL)chaperonin GroEL  [2] (data from MRSA252)
    SACOL0461(guaA)GMP synthase  [2] (data from MRSA252)
    SACOL0460(guaB)inosine-5'-monophosphate dehydrogenase  [2] (data from MRSA252)
    SACOL1513(hup)DNA-binding protein HU  [2] (data from MRSA252)
    SACOL1727(infC)translation initiation factor IF-3  [2] (data from MRSA252)
    SACOL0222(ldh1)L-lactate dehydrogenase  [2] (data from MRSA252)
    SACOL2623(mqo2)malate:quinone oxidoreductase  [2] (data from MRSA252)
    SACOL1102(pdhA)pyruvate dehydrogenase complex E1 component subunit alpha  [2] (data from MRSA252)
    SACOL1005(pepF)oligoendopeptidase F  [2] (data from MRSA252)
    SACOL0204(pflB)formate acetyltransferase  [2] (data from MRSA252)
    SACOL1091(ptsH)phosphocarrier protein HPr  [2] (data from MRSA252)
    SACOL1745(pyk)pyruvate kinase  [2] (data from MRSA252)
    SACOL0584(rplA)50S ribosomal protein L1  [2] (data from MRSA252)
    SACOL2236(rplB)50S ribosomal protein L2  [2] (data from MRSA252)
    SACOL2238(rplD)50S ribosomal protein L4  [2] (data from MRSA252)
    SACOL2224(rplF)50S ribosomal protein L6  [2] (data from MRSA252)
    SACOL0585(rplJ)50S ribosomal protein L10  [2] (data from MRSA252)
    SACOL0583(rplK)50S ribosomal protein L11  [2] (data from MRSA252)
    SACOL2232(rplP)50S ribosomal protein L16  [2] (data from MRSA252)
    SACOL2212(rplQ)50S ribosomal protein L17  [2] (data from MRSA252)
    SACOL1257(rplS)50S ribosomal protein L19  [2] (data from MRSA252)
    SACOL1702(rplU)50S ribosomal protein L21  [2] (data from MRSA252)
    SACOL2237(rplW)50S ribosomal protein L23  [2] (data from MRSA252)
    SACOL1726(rpmI)50S ribosomal protein L35  [2] (data from MRSA252)
    SACOL1516(rpsA)30S ribosomal protein S1  [2] (data from MRSA252)
    SACOL2233(rpsC)30S ribosomal protein S3  [2] (data from MRSA252)
    SACOL1769(rpsD)30S ribosomal protein S4  [2] (data from MRSA252)
    SACOL2222(rpsE)30S ribosomal protein S5  [2] (data from MRSA252)
    SACOL0437(rpsF)30S ribosomal protein S6  [2] (data from MRSA252)
    SACOL0592(rpsG)30S ribosomal protein S7  [2] (data from MRSA252)
    SACOL2206(rpsI)30S ribosomal protein S9  [2] (data from MRSA252)
    SACOL2240(rpsJ)30S ribosomal protein S10  [2] (data from MRSA252)
    SACOL2214(rpsK)30S ribosomal protein S11  [2] (data from MRSA252)
    SACOL2215(rpsM)30S ribosomal protein S13  [2] (data from MRSA252)
    SACOL1292(rpsO)30S ribosomal protein S15  [2] (data from MRSA252)
    SACOL2230(rpsQ)30S ribosomal protein S17  [2] (data from MRSA252)
    SACOL0439(rpsR)30S ribosomal protein S18  [2] (data from MRSA252)
    SACOL2235(rpsS)30S ribosomal protein S19  [2] (data from MRSA252)
    SACOL1642(rpsT)30S ribosomal protein S20  [2] (data from MRSA252)
    SACOL1722(tig)trigger factor  [2] (data from MRSA252)
    SACOL03035'-nucleotidase  [2] (data from MRSA252)
    SACOL0564pyridoxal biosynthesis lyase PdxS  [2] (data from MRSA252)
    SACOL0587hypothetical protein  [2] (data from MRSA252)
    SACOL0707dihydroxyacetone kinase subunit DhaK  [2] (data from MRSA252)
    SACOL0731LysR family transcriptional regulator  [2] (data from MRSA252)
    SACOL0742hypothetical protein  [2] (data from MRSA252)
    SACOL1098hypothetical protein  [2] (data from MRSA252)
    SACOL1294metallo-beta-lactamase  [2] (data from MRSA252)
    SACOL1670hypothetical protein  [2] (data from MRSA252)
    SACOL1753universal stress protein  [2] (data from MRSA252)
    SACOL1952ferritins family protein  [2] (data from MRSA252)
    SACOL1994ABC transporter ATP-binding protein  [2] (data from MRSA252)
    SACOL2173alkaline shock protein 23  [2] (data from MRSA252)
    SACOL2561hydroxymethylglutaryl-CoA synthase  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Zur* (repression) regulon
    Zur*(TF)important in Zinc homeostasis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 2.35 2.36 2.37 2.38 2.39 2.40 2.41 2.42 2.43 2.44 2.45 2.46 2.47 2.48 2.49 2.50 2.51 2.52 2.53 2.54 2.55 2.56 2.57 2.58 2.59 2.60 2.61 2.62 2.63 2.64 2.65 2.66 2.67 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL1370&oldid=69011"