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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0709 [new locus tag: SA_RS04035 ]
  • pan locus tag?: SAUPAN002664000
  • symbol: prfB
  • pan gene symbol?: prfB
  • synonym:
  • product: peptide chain release factor 2

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0709 [new locus tag: SA_RS04035 ]
  • symbol: prfB
  • product: peptide chain release factor 2
  • replicon: chromosome
  • strand: +
  • coordinates: 809375..810367
  • length: 993
  • essential: yes DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCAGAACCTAATTTTTGGGATAACCAAACGAAAGCGCAAGATATTATAGATAAAAAT
    AATGCGTTAAAAGCAATAGTTAATGGTTATAAAACACTACAAGCAGAAGTAGATGACATG
    GATGCTACTTGGGATTTATTACAAGAAGAATTTGATGAAGAAATGAAAGAAGACTTAGAG
    CAAGAGGTCATTAATTTTAAGGCTAAAGTGGATGAATACGAATTGCAATTATTATTAGAT
    GGGCCTCACGATGCCAATAACGCAATTCTAGAGTTACATCCTGGTGCAGGTGGCACGGAG
    TCTCAAGATTGGGCTAATATGCTATTTAGAATGTATCAACGTTATTGTGAGAAGAAAGGC
    TTTAAAGTTGAAACTGTTGATTATCTACCTGGGGATGAAGCGGGGATTAAAAGTGTAACA
    TTGCTCATCAAAGGGCATAATGCTTATGGTTATTTAAAAGCTGAAAAAGGTGTACACCGA
    CTAGTACGAATTTCTCCATTTGATTCATCAGGACGTCGTCATACATCATTTGCATCATGC
    GACGTTATTCCAGATTTTAATAATGATGAAATAGAGATTGAAATCAATCCGGATGATATT
    ACAGTTGATACATTCAGAGCTTCTGGTGCAGGTGGTCAGCATATTAACAAAACTGAATCG
    GCAATACGAATTACCCACCACCCCTCAGGTATAGTTGTTAATAACCAAAATGAACGTTCT
    CAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAA
    TTGGAAGAGCAGGCACGTGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGG
    GGAAGCCAAATTAGATCATATGTTTTCCATCCATACTCAATGGTGAAAGATCATCGTACG
    AACGAAGAAACAGGTAAGGTTGATGCAGTGATGGATGGAGACATTGGACCATTTATCGAA
    TCATATTTAAGACAGACAATGTCGCACGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    993

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0709 [new locus tag: SA_RS04035 ]
  • symbol: PrfB
  • description: peptide chain release factor 2
  • length: 330
  • theoretical pI: 4.70098
  • theoretical MW: 37616.8
  • GRAVY: -0.699697

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesisTranslation factorspeptide chain release factor 2 (TIGR00020; HMM-score: 506.2)
    Genetic information processingProtein synthesisTranslation factorspeptide chain release factor 1 (TIGR00019; HMM-score: 298.6)
    Genetic information processingProtein synthesisTranslation factorsputative peptide chain release factor H (TIGR03072; HMM-score: 120.6)
  • TheSEED:  
    Protein MetabolismProtein biosynthesisProgrammed frameshift Peptide chain release factor 2 
    Protein biosynthesisProgrammed frameshift programmed frameshift-containing 
    Protein biosynthesisTranslation termination factors bacterial Peptide chain release factor 2 
    CBSS-393121.3.peg.2760 Peptide chain release factor 2 
  • PFAM:
    no clan definedPCRF; PCRF domain (PF03462; HMM-score: 208)
    RF (CL0337) RF-1; RF-1 domain (PF00472; HMM-score: 146.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA1984(asp23)alkaline shock protein 23  [1] (data from MRSA252)
    SA1517(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    SA1409(dnaK)molecular chaperone DnaK  [1] (data from MRSA252)
    SA0869(fabI)enoyl-ACP reductase  [1] (data from MRSA252)
    SA1029(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    SA0505(fus)elongation factor G  [1] (data from MRSA252)
    SA0727(gap)glyceraldehyde-3-phosphate dehydrogenase  [1] (data from MRSA252)
    SA1305(hu)DNA-binding protein II  [1] (data from MRSA252)
    SA2074(modA)molybdate-binding protein  [1] (data from MRSA252)
    SA1244(odhB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA0944(pdhB)pyruvate dehydrogenase E1 component subunit beta  [1] (data from MRSA252)
    SA0945(pdhC)branched-chain alpha-keto acid dehydrogenase E2 subunit  [1] (data from MRSA252)
    SA0946(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SA0218(pflB)formate acetyltransferase  [1] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA0496(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SA2044(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    SA2047(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SA2046(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    SA0497(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SA0498(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    SA2017(rplM)50S ribosomal protein L13  [1] (data from MRSA252)
    SA2029(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SA2032(rplR)50S ribosomal protein L18  [1] (data from MRSA252)
    SA1084(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SA1473(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SA2042(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    SA2045(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SA2041(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SAS052(rpsD)30S ribosomal protein S4  [1] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SA2016(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    SA2024(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [1] (data from MRSA252)
    SA0223hypothetical protein  [1] (data from MRSA252)
    SA0224hypothetical protein  [1] (data from MRSA252)
    SA0627hypothetical protein  [1] (data from MRSA252)
    SA0802hypothetical protein  [1] (data from MRSA252)
    SA1532hypothetical protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.101
    • Ymax_pos: 63
    • Cmax: 0.113
    • Cmax_pos: 36
    • Smax: 0.121
    • Smax_pos: 55
    • Smean: 0.08
    • D: 0.093
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAEPNFWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEVINFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFASCDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQNERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]