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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02647
  • pan locus tag?: SAUPAN005877000
  • symbol: SAOUHSC_02647
  • pan gene symbol?: mqo1
  • synonym:
  • product: malate:quinone oxidoreductase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02647
  • symbol: SAOUHSC_02647
  • product: malate:quinone oxidoreductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2431151..2432629
  • length: 1479
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAACACAACATAGCAAAACAGATGTCATCTTAATTGGTGGCGGTATTATGAGTGCA
    ACATTAGGAACATTACTTAAAGAATTATCACCTGAGAAAAATATTAAAGTGTTTGAAAAA
    TTAGCACAACCTGGCGAAGAGAGTTCAAATGTATGGAATAATGCCGGTACAGGGCATTCA
    GCACTTTGCGAGTTGAACTATACAAAAGAAGGTAAGGATGGCACAGTTGATTGTAGTAAA
    GCAATTAAGATAAATGAGCAGTACCAAATTTCAAAACAGTTTTGGGCATATTTAGTTAAA
    ACAGGACAATTAGATAACCCAGATCGCTTTATTCAAGCGGTGCCACACATGAGTTTTGTC
    ATTGGCGAAGATAATGTAGCTTTTATAAAAAGTCGTGTTGCAACGTTAAAGAAAAGTATT
    TTATTCGAAAAAATGAAATTATCGCAAGATGAAGAAGAAATGAAATCTTGGGTACCGTTA
    ATGATTGAAGGTCGTAAGTCTGATGAACCAATTGCTTTAACTTATGATGAAACTGGTACA
    GATGTTAACTTTGGTGCGTTAACTGCAAAGTTATTTGATAATTTAGAGCAACGTGGTGTG
    GGAATTCAATATAAGCAGAATGTATTAGACATCAAGAAACAGAAATCTGGGGTATGGCTA
    GTTAAAGTTAAAGATTTAGAAACTAATGAAACGACAACATATGAATCTGATTTTGTATTT
    ATTGGTGCTGGCGGTGCGAGTTTACCATTACTCCAAAAGACTGGGATTAAACAATCAAAA
    CATATTGGTGGTTTCCCGGTAAGTGGATTATTCCTGCGCTGTACAAATCAAGAAGTGATT
    GATCGTCATCATGCTAAAGTGTACGGAAAAGCAGCAGTGGGTGCGCCACCAATGTCAGTG
    CCGCACTTAGATACACGTTTTGTAGACGGCAAGCGTTCATTGTTATTTGGTCCATTTGCA
    GGTTTCTCACCTAAATTTTTAAAAACAGGTTCACATATGGATTTAATTAAATCGGTTAAA
    CCAAATAATATCGTGACGATGTTATCTGCAGGTATCAAAGAAATGAGTCTTACGAAGTAT
    TTAGTGTCACAATTGATGTTATCTAATGATGAGCGTATGGATGATTTAAGAGTCTTTTTC
    CCAAATGCTAAAAATGAAGATTGGGAAGTGATTACAGCAGGGCAACGTGTCCAAGTAATC
    AAGGATACTGAGGATTCTAAAGGTAACTTACAATTTGGTACTGAAGTTATTACGTCAGAT
    GATGGCACATTAGCTGCATTACTTGGTGCATCACCTGGTGCGTCAACAGCTGTAGATATT
    ATGTTTGATGTTTTACAGAGATGCTATCGTGATGAATTCAAAGGATGGGAACCAAAGATT
    AAAGAAATGGTGCCGTCATTTGGTTATCGCTTAACAGATCATGAGGATTTATATCATAAA
    ATTAATGAAGAAGTAACTAAGTATTTACAAGTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1479

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02647
  • symbol: SAOUHSC_02647
  • description: malate:quinone oxidoreductase
  • length: 492
  • theoretical pI: 6.68621
  • theoretical MW: 54813.4
  • GRAVY: -0.321748

Function[edit source | edit]

  • reaction:
    EC 1.1.5.4?  ExPASy
    Malate dehydrogenase (quinone)(S)-malate + a quinone = oxaloacetate + reduced quinone
  • TIGRFAM:
    MetabolismEnergy metabolismTCA cyclemalate dehydrogenase (acceptor) (TIGR01320; EC 1.1.5.4; HMM-score: 709.8)
    and 4 more
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 17.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherC-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 16.4)
    Unknown functionEnzymes of unknown specificityflavoprotein, HI0933 family (TIGR00275; HMM-score: 15)
    MetabolismEnergy metabolismElectron transportthioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 12.4)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismTCA Cycle Malate:quinone oxidoreductase (EC 1.1.5.4) 
  • PFAM:
    NADP_Rossmann (CL0063) Mqo; Malate:quinone oxidoreductase (Mqo) (PF06039; HMM-score: 769.3)
    and 5 more
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 36.9)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 16.8)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 16.2)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 12.6)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 10.6)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.53
    • Cellwall Score: 8.75
    • Extracellular Score: 0.7
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.203
    • Ymax_pos: 38
    • Cmax: 0.173
    • Cmax_pos: 38
    • Smax: 0.314
    • Smax_pos: 19
    • Smean: 0.233
    • D: 0.215
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQPGEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTGQLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSILFEKMKLSQDEEEMKSWVPLMIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVGIQYKQNVLDIKKQKSGVWLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTNQEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMDLIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVITAGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYRDEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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