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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05710 [old locus tag: SACOL1118 ]
  • pan locus tag?: SAUPAN003336000
  • symbol: SACOL_RS05710
  • pan gene symbol?: typA
  • synonym:
  • product: translational GTPase TypA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS05710 [old locus tag: SACOL1118 ]
  • symbol: SACOL_RS05710
  • product: translational GTPase TypA
  • replicon: chromosome
  • strand: +
  • coordinates: 1127325..1129172
  • length: 1848
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (1127325..1129172) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    361
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    481
    541
    601
    661
    721
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    841
    901
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    1021
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    1141
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    1801
    ATGACTAATAAAAGAGAAGATGTCCGCAATATAGCAATTATTGCTCACGTTGACCATGGT
    AAAACAACTTTAGTAGATGAGTTGTTAAAACAATCTGGTATATTCAGAGAAAATGAACAT
    GTCGATGAACGTGCAATGGACTCTAACGATATCGAAAGAGAGCGTGGAATTACGATTCTA
    GCCAAAAATACGGCTGTTGATTATAAAGGTACACGTATTAATATTTTGGATACACCAGGA
    CATGCAGACTTTGGTGGAGAAGTAGAACGTATTATGAAAATGGTTGATGGGGTTGTCTTA
    GTAGTAGATGCGTATGAAGGTACAATGCCTCAAACACGTTTTGTACTTAAAAAAGCGCTA
    GAACAAAACCTGAAACCTGTTGTTGTTGTTAATAAAATTGATAAACCATCAGCACGTCCA
    GAGGGTGTTGTAGATGAAGTTTTAGATTTATTTATTGAATTAGAAGCAAACGATGAACAA
    TTAGAATTCCCTGTTGTTTATGCTTCAGCAGTAAATGGTACAGCTAGCTTAGATCCTGAA
    AAGCAAGATGATAATTTACAATCATTATATGAAACAATTATTGATTATGTACCAGCTCCA
    ATTGATAACAGTGATGAGCCATTACAATTCCAAGTAGCATTGTTGGACTACAATGATTAT
    GTTGGACGTATTGGTATTGGTCGTGTATTCAGAGGTAAAATGCGTGTCGGAGATAATGTA
    TCACTAATTAAATTAGACGGTACAGTGAAAAACTTCCGTGTAACTAAAATCTTTGGTTAC
    TTTGGATTAAAACGTTTAGAAATTGAAGAAGCACAAGCTGGAGATTTAATTGCTGTTTCA
    GGTATGGAAGACATTAATGTTGGTGAAACTGTAACACCACATGACCATCAAGAAGCATTG
    CCAGTTCTACGTATTGATGAGCCTACTCTTGAAATGACATTTAAAGTTAACAATTCTCCA
    TTTGCTGGCCGTGAAGGTGACTTTGTAACAGCACGTCAAATTCAAGAACGTTTAAATCAA
    CAATTAGAAACAGATGTATCTTTGAAAGTTTCTAACACAGATTCTCCAGATACATGGGTA
    GTTGCTGGTCGCGGTGAATTGCATTTATCAATCCTTATTGAAAATATGCGTCGTGAAGGT
    TATGAATTACAAGTTTCAAAACCACAAGTAATTATTAAAGAAATAGATGGTGTAATGTGT
    GAACCATTTGAACGTGTGCAATGTGAAGTGCCACAAGAAAATGCAGGTGCTGTTATTGAA
    TCATTAGGTGCACGTAAAGGTGAAATGGTTGATATGACTACAACTGATAATGGACTTACA
    CGTTTAATCTTTAATGTACCGGCTCGTGGTATGATTGGTTATACGACTGAATTTATGTCA
    ATGACAAGAGGTTACGGTATTATTAACCATACATTTGAAGAATTTAGACCACGTATTAAA
    GCACAAATTGGCGGTCGTCGTAATGGTGCATTAATTTCAATGGATCAAGGTTCTGCAAGT
    ACTTATGCCATTTTGGGACTTGAAGATAGAGGTGTAAACTTCATGGAACCTGGTACTGAA
    GTTTATGAAGGTATGATTGTTGGTGAACATAATCGTGAAAATGATTTAACTGTTAACATC
    ACTAAAACAAAACATCAAACTAACGTACGTTCTGCAACGAAAGACCAAACACAAACAATG
    AATAGACCGCGTATTCTAACATTGGAAGAAGCGTTACAATTCATTAATGATGATGAACTT
    GTTGTGGTTACACCAGAAAGTATACGTTTAAGAAAGAAAATTTTAAACAAAAATGTTCGT
    GAAAAAGAAGCAAAGCGTATCAAACAAATGATGCAAGAAAACGAATAA
    60
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    1848

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS05710 [old locus tag: SACOL1118 ]
  • symbol: SACOL_RS05710
  • description: translational GTPase TypA
  • length: 615
  • theoretical pI: 4.68551
  • theoretical MW: 69165
  • GRAVY: -0.405203

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesAdaptations to atypical conditionsGTP-binding protein TypA/BipA (TIGR01394; HMM-score: 939)
    Genetic information processingProtein synthesisTranslation factorsGTP-binding protein TypA/BipA (TIGR01394; HMM-score: 939)
    Signal transductionRegulatory functionsOtherGTP-binding protein TypA/BipA (TIGR01394; HMM-score: 939)
    Genetic information processingProtein synthesisTranslation factorstranslation elongation factor G (TIGR00484; HMM-score: 220.7)
    Genetic information processingProtein synthesisTranslation factorstranslation elongation factor aEF-2 (TIGR00490; HMM-score: 217.2)
    Unknown functionGeneralelongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 209.7)
    Genetic information processingProtein synthesisTranslation factorstranslation elongation factor Tu (TIGR00485; HMM-score: 100.1)
    Genetic information processingProtein synthesisTranslation factorspeptide chain release factor 3 (TIGR00503; HMM-score: 92.9)
    Unknown functionGeneralsmall GTP-binding protein domain (TIGR00231; HMM-score: 84.9)
    Genetic information processingProtein synthesisTranslation factorsselenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 75.1)
    Genetic information processingProtein synthesisTranslation factorstranslation initiation factor IF-2 (TIGR00487; HMM-score: 69.8)
    Genetic information processingProtein synthesisTranslation factorstranslation elongation factor EF-1, subunit alpha (TIGR00483; HMM-score: 67.8)
    MetabolismCentral intermediary metabolismSulfur metabolismsulfate adenylyltransferase, large subunit (TIGR02034; EC 2.7.7.4; HMM-score: 48.7)
    Genetic information processingProtein synthesisTranslation factorstranslation initiation factor aIF-2 (TIGR00491; HMM-score: 44.2)
    translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 39.8)
    Genetic information processingProtein synthesisOtherribosome-associated GTPase EngA (TIGR03594; HMM-score: 26.2)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 13.6)
    Genetic information processingProtein fateProtein modification and repair[FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 13.5)
    exopolysaccharide/PEP-CTERM locus tyrosine autokinase (TIGR03018; EC 2.7.10.2; HMM-score: 13.3)
    Genetic information processingProtein synthesisOtherribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 13)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsferrous iron transport protein B (TIGR00437; HMM-score: 12.2)
  • TheSEED:
  • PFAM:
    P-loop_NTPase (CL0023) GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 193.4)
    EF-G_C (CL0437) EFG_C; Elongation factor G C-terminus (PF00679; HMM-score: 78.6)
    EFTPs (CL0575) GTP_EFTU_D2; Elongation factor Tu domain 2 (PF03144; HMM-score: 41.2)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 31.7)
    EF-G_C (CL0437) EFG_II; Elongation Factor G, domain II (PF14492; HMM-score: 30.2)
    P-loop_NTPase (CL0023) FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 20.3)
    Arf; ADP-ribosylation factor family (PF00025; HMM-score: 19.8)
    Septin; Septin (PF00735; HMM-score: 17.4)
    SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 13.6)
    Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 13.6)
    Ras; Ras family (PF00071; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.113
    • Ymax_pos: 44
    • Cmax: 0.11
    • Cmax_pos: 42
    • Smax: 0.178
    • Smax_pos: 43
    • Smean: 0.099
    • D: 0.108
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446104801 NCBI
  • RefSeq: WP_000182656 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDERAMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVVDAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQLEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYNDYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDLIAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQIQERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVIIKEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARGMIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLEDRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRILTLEEALQFINDDELVVVTPESIRLRKKILNKNVREKEAKRIKQMMQENE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS05710 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]