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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06485 [old locus tag: NWMN_1150 ]
  • pan locus tag?: SAUPAN003529000
  • symbol: NWMN_RS06485
  • pan gene symbol?: trmD
  • synonym:
  • product: tRNA (guanosine(37)-N1)-methyltransferase TrmD

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS06485 [old locus tag: NWMN_1150 ]
  • symbol: NWMN_RS06485
  • product: tRNA (guanosine(37)-N1)-methyltransferase TrmD
  • replicon: chromosome
  • strand: +
  • coordinates: 1260658..1261395
  • length: 738
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1260658..1261395) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1150

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAAATTGATTATTTAACTTTATTTCCTGAAATGTTTGATGGTGTTTTAAATCATTCA
    ATTATGAAACGTGCCCAAGAAAACAATAAATTACAAATCAATACGGTTAATTTTAGAGAT
    TATGCAATTAACAAGCACAACCAAGTAGATGATTATCCGTATGGTGGCGGACAAGGTATG
    GTGTTAAAGCCTGAACCTGTTTTTAATGCGATGGAAGACTTAGATGTCACAGAACAAACA
    CGCGTTATTTTAATGTGTCCACAAGGCGAGCCATTTTCACATCAGAAAGCTGTTGAATTA
    AGCAAGGCCGACCACATCGTTTTCATATGCGGACATTATGAAGGTTACGATGAACGTATC
    CGAACACATCTTGTCACAGATGAAATATCAATGGGTGACTATGTTTTAACTGGTGGAGAA
    TTGCCAGCGATGACCATGACTGATGCTATTGTTAGACTGATTCCAGGTGTTTTAGGTAAT
    GAACAGTCACATCAAGACGATTCATTTTCAGATGGGTTATTAGAGTTTCCGCAATATACA
    CGTCCGCGTGAATTTAAGGGTCTAACAGTTCCAGATGTTTTATTGTCTGGAAATCATGCC
    AATATTGATGCATGGAGACATGAGCAAAAGTTGATCCGCACATATAATAAAAGACCTGAC
    TTAATTGAAAAATATCCATTAACTAATGCAGATAAGCAAATATTAGAAAGATATAAAATA
    GGATTGAAAAAAGGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    738

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS06485 [old locus tag: NWMN_1150 ]
  • symbol: NWMN_RS06485
  • description: tRNA (guanosine(37)-N1)-methyltransferase TrmD
  • length: 245
  • theoretical pI: 5.57373
  • theoretical MW: 28055.7
  • GRAVY: -0.513469

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.228?  ExPASy
    tRNA (guanine37-N1)-methyltransferase S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine(37)-N(1))-methyltransferase (TIGR00088; EC 2.1.1.228; HMM-score: 334)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    SPOUT (CL0098) tRNA_m1G_MT; tRNA (Guanine-1)-methyltransferase (PF01746; HMM-score: 238.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012706
    • TAT(Tat/SPI): 0.000519
    • LIPO(Sec/SPII): 0.001292
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGNEQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNADKQILERYKIGLKKG

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 1.108 1.109 1.110 1.111 1.112 1.113 1.114 1.115 1.116 1.117 1.118 1.119 1.120 1.121 1.122 1.123 1.124 1.125 1.126 1.127 1.128 1.129 1.130 1.131 1.132 1.133 1.134 1.135 1.136 1.137 1.138 1.139 1.140 1.141 1.142 1.143 1.144 1.145 1.146 1.147 1.148 1.149 1.150 1.151 1.152 1.153 1.154 1.155 1.156 1.157 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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