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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06675 [old locus tag: NWMN_1184 ]
  • pan locus tag?: SAUPAN003576000
  • symbol: NWMN_RS06675
  • pan gene symbol?: rnjB
  • synonym:
  • product: ribonuclease J 2

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS06675 [old locus tag: NWMN_1184 ]
  • symbol: NWMN_RS06675
  • product: ribonuclease J 2
  • replicon: chromosome
  • strand: +
  • coordinates: 1304379..1306052
  • length: 1674
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_009641 (1304379..1306052) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_1184

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    TTGAGTTTAATAAAGAAAAAGAATAAAGATATTCGCATTATACCATTAGGCGGTGTTGGC
    GAAATTGCTAAAAATATGTATATCGTTGAAGTAGACGATGAAATGTTTATGTTAGATGCT
    GGACTTATGTTTCCAGAAGACGAAATGCTAGGTATTGATATTGTTATACCAGACATTTCA
    TACGTACTTGAAAATAAAGATAAATTGAAGGGTATATTCCTTACACACGGACATGAGCAC
    GCGATTGGTGCAGTGAGTTATGTTTTAGAACAATTAGATGCACCAGTATATGGATCTAAA
    TTGACAATAGCGTTAATTAAAGAAAATATGAAAGCCCGTAATATTGATAAAAAAGTTCGC
    TACTATACAGTTAATAATGATTCAATTATGAGATTCAAAAACGTGAATATTAGTTTCTTT
    AATACGACACACAGTATTCCTGATAGTTTAGGTGTTTGTATTCACACTTCATATGGTGCC
    ATTGTGTATACAGGTGAATTTAAGTTTGACCAAAGTTTACATGGACATTATGCACCAGAT
    ATTAAACGTATGGCAGAGATTGGTGAAGAAGGCGTATTTGTCTTAATCAGTGATTCTACT
    GAGGCAGAGAAACCTGGATATAATACTCCGGAAAATGTGATTGAACATCATATGTATGAT
    GCTTTTGCAAAAGTGCGAGGTCGCTTGATAGTTTCATGTTATGCTTCGAACTTTATACGT
    ATTCAGCAAGTTTTAAATATTGCTAGCAAGCTAAATCGTAAAGTGTCATTTTTAGGAAGA
    TCACTTGAAAGTTCATTTAATATTGCTCGTAAAATGGGGTATTTCGACATTCCTAAAGAT
    TTGCTAATTCCTATAACAGAAGTTGATAATTATCCTAAAAATGAAGTGATAATTATAGCT
    ACTGGTATGCAAGGAGAACCTGTAGAAGCCTTAAGTCAAATGGCGCAACATAAGCATAAA
    ATTATGAATATCGAAGAAGGCGATTCTGTATTTTTAGCAATTACGGCTTCTGCTAATATG
    GAAGTTATCATTGCGAATACATTAAATGAGCTTGTACGTGCTGGCGCACATATTATTCCA
    AATAACAAAAAGATTCATGCTTCAAGTCATGGTTGCATGGAAGAATTAAAAATGATGATT
    AATATTATGAAACCTGAATACTTTATTCCTGTACAAGGTGAATTTAAAATGCAGATAGCA
    CATGCGAAGCTAGCAGCTGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAA
    GGAGATGTCATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGA
    AATATTTTAATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGT
    CATCTTTTAGCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGA
    CGTATAGCTGCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAA
    GACTTATTACGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGTTTACAAGAA
    AAACGCATAGAATGGTCTGAAATTAAACAAAATATGCGTGATCAAATTAGTAAACTATTA
    TTCGAAAGTACAAAACGTCGTCCTATGATTATTCCAGTAATTTCTGAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1674

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_RS06675 [old locus tag: NWMN_1184 ]
  • symbol: NWMN_RS06675
  • description: ribonuclease J 2
  • length: 557
  • theoretical pI: 6.10952
  • theoretical MW: 62603.1
  • GRAVY: -0.0983842

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Transcription Degradation of RNA beta-CASP ribonuclease, RNase J family (TIGR00649; EC 3.1.-.-; HMM-score: 606)
    and 2 more
    arCOG00543 universal archaeal KH-domain/beta-lactamase-domain protein (TIGR03675; HMM-score: 26.3)
    Cellular processes Cellular processes Detoxification hydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 16.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    RMMBL_DRMBL (CL0398) RMMBL; Zn-dependent metallo-hydrolase RNA specificity domain (PF07521; HMM-score: 44.4)
    and 4 more
    Metallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 29.7)
    Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 22.6)
    ATP_synthase (CL0255) vATP-synt_E; ATP synthase (E/31 kDa) subunit (PF01991; HMM-score: 15.1)
    HTH (CL0123) HTH_10; HTH DNA binding domain (PF04967; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001198
    • TAT(Tat/SPI): 0.000054
    • LIPO(Sec/SPII): 0.000176
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMFPEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLTIALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGAIVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHMYDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFDIPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAITASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQGEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIGDVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEEKVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 1.108 1.109 1.110 1.111 1.112 1.113 1.114 1.115 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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