From AureoWiki
Jump to: navigation, search

NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1168 [new locus tag: SAUSA300_RS06320 ]
  • pan locus tag?: SAUPAN003576000
  • symbol: SAUSA300_1168
  • pan gene symbol?: rnjB
  • synonym:
  • product: RNA-metabolising metallo-beta-lactamase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_1168 [new locus tag: SAUSA300_RS06320 ]
  • symbol: SAUSA300_1168
  • product: RNA-metabolising metallo-beta-lactamase
  • replicon: chromosome
  • strand: +
  • coordinates: 1284342..1286066
  • length: 1725
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGAATAGTGAGTTTATATATGGACGGGTAACAAATTTAGGAGGTAAGATTTTGAGTTTA
    ATAAAGAAAAAGAATAAAGATATTCGCATTATACCATTAGGCGGTGTTGGCGAAATTGCT
    AAAAATATGTATATCGTTGAAGTAGACGATGAAATGTTTATGTTAGATGCTGGACTTATG
    TTTCCAGAAGACGAAATGCTAGGTATTGATATTGTTATACCAGACATTTCATACGTACTT
    GAAAATAAAGATAAATTGAAGGGTATATTCCTTACACACGGACATGAGCACGCGATTGGT
    GCAGTGAGTTATGTTTTAGAACAATTAGATGCACCAGTATATGGATCTAAATTGACAATA
    GCGTTAATTAAAGAAAATATGAAAGCCCGTAATATTGATAAAAAAGTTCGCTACTATACA
    GTTAATAATGATTCAATTATGAGATTCAAAAACGTGAATATTAGTTTCTTTAATACGACA
    CACAGTATTCCTGATAGTTTAGGTGTTTGTATTCACACTTCATATGGTGCCATTGTGTAT
    ACAGGTGAATTTAAGTTTGACCAAAGTTTACATGGACATTATGCACCAGATATTAAACGT
    ATGGCAGAGATTGGTGAAGAAGGCGTATTTGTCTTAATCAGTGATTCTACTGAGGCAGAG
    AAACCTGGATATAATACTCCGGAAAATGTGATTGAACATCATATGTATGATGCTTTTGCA
    AAAGTGCGAGGTCGCTTGATAGTTTCATGTTATGCTTCGAACTTTATACGTATTCAGCAA
    GTTTTAAATATTGCTAGCAAGCTAAATCGTAAAGTGTCATTTTTAGGAAGATCACTTGAA
    AGTTCATTTAATATTGCTCGTAAAATGGGGTATTTCGACATTCCTAAAGATTTGCTAATT
    CCTATAACAGAAGTTGATAATTATCCTAAAAATGAAGTGATAATTATAGCTACTGGTATG
    CAAGGAGAACCTGTAGAAGCCTTAAGTCAAATGGCGCAACATAAGCATAAAATTATGAAT
    ATCGAAGAAGGCGATTCTGTATTTTTAGCAATTACGGCTTCTGCTAATATGGAAGTTATC
    ATTGCGAATACATTAAATGAGCTTGTACGTGCTGGCGCACATATTATTCCAAATAACAAA
    AAGATTCATGCTTCAAGTCATGGTTGCATGGAAGAATTAAAAATGATGATTAATATTATG
    AAACCTGAATACTTTATTCCTGTACAAGGTGAATTTAAAATGCAGATAGCACATGCGAAG
    CTAGCAGCTGAAGCAGGTGTTGCACCAGAAAAGATTTTCCTTGTGGAAAAAGGAGATGTC
    ATTAATTACAACGGTAAAGATATGATATTAAATGAAAAGGTAAATTCAGGAAATATTTTA
    ATAGATGGCATTGGTATTGGGGATGTAGGAAATATCGTGTTGAGAGACCGTCATCTTTTA
    GCAGAAGATGGTATCTTTATTGCTGTTGTAACGTTAGATCCTAAAAATAGACGTATAGCT
    GCGGGACCTGAAATTCAATCTCGTGGGTTTGTATATGTACGTGAAAGTGAAGACTTATTA
    CGTGAAGCAGAAGAGAAAGTACGTGAAATAGTAGAGGCTGGTTTACAAGAAAAACGCATA
    GAATGGTCTGAAATTAAACAAAATATGCGTGATCAAATTAGTAAACTATTATTCGAAAGT
    ACAAAACGTCGTCCTATGATTATTCCAGTAATTTCTGAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1725

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_1168 [new locus tag: SAUSA300_RS06320 ]
  • symbol: SAUSA300_1168
  • description: RNA-metabolising metallo-beta-lactamase
  • length: 574
  • theoretical pI: 6.21782
  • theoretical MW: 64466.3
  • GRAVY: -0.0942509

Function[edit source | edit]

  • reaction:
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processingTranscriptionDegradation of RNAbeta-CASP ribonuclease, RNase J family (TIGR00649; EC 3.1.-.-; HMM-score: 605.8)
    and 2 more
    arCOG00543 universal archaeal KH-domain/beta-lactamase-domain protein (TIGR03675; HMM-score: 30.3)
    Cellular processesCellular processesDetoxificationhydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 16.8)
  • TheSEED:  
    Bacterial RNA-metabolizing Zn-dependent hydrolases Ribonuclease J2 (endoribonuclease in RNA processing) 
  • PFAM:
    RMMBL_DRMBL (CL0398) RMMBL; Zn-dependent metallo-hydrolase RNA specificity domain (PF07521; HMM-score: 44.4)
    and 4 more
    Metallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 29.6)
    Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 22.5)
    ATP_synthase (CL0255) vATP-synt_E; ATP synthase (E/31 kDa) subunit (PF01991; HMM-score: 15.1)
    HTH (CL0123) HTH_10; HTH DNA binding domain (PF04967; HMM-score: 13.5)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.103
    • Ymax_pos: 11
    • Cmax: 0.108
    • Cmax_pos: 27
    • Smax: 0.154
    • Smax_pos: 2
    • Smean: 0.103
    • D: 0.103
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNSEFIYGRVTNLGGKILSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMFPEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLTIALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGAIVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHMYDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFDIPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAITASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQGEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIGDVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEEKVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 1.108 1.109 1.110 1.111 1.112 1.113 1.114 1.115 1.116 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]