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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01251
  • pan locus tag?: SAUPAN003575000
  • symbol: SAOUHSC_01251
  • pan gene symbol?: pnpA
  • synonym:
  • product: polynucleotide phosphorylase/polyadenylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01251
  • symbol: SAOUHSC_01251
  • product: polynucleotide phosphorylase/polyadenylase
  • replicon: chromosome
  • strand: +
  • coordinates: 1202091..1204187
  • length: 2097
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTCTCAAGAAAAGAAAGTTTTTAAAACTGAATGGGCAGGAAGATCTTTAACGATTGAA
    ACAGGGCAATTAGCTAAACAAGCAAATGGCGCTGTATTGGTTCGTTATGGAGATACAGTC
    GTGTTATCGACGGCAACTGCATCAAAAGAACCTCGTGATGGAGATTTCTTCCCATTAACA
    GTGAACTATGAAGAAAAAATGTACGCTGCGGGTAAAATTCCTGGTGGATTTAAAAAGAGA
    GAAGGACGTCCTGGTGACGATGCAACATTAACTGCGCGATTAATTGATAGACCAATTAGA
    CCTTTATTCCCTAAAGGATATAAGCATGATGTTCAAATTATGAACATGGTATTAAGTGCA
    GATCCTGATTGTTCACCACAAATGGCTGCAATGATTGGTTCATCTATGGCGCTTAGTGTG
    TCGGATATTCCATTCCAAGGGCCAATCGCCGGTGTAAATGTGGGTTATATTGACGGTAAA
    TATATCATTAACCCAACAGTAGAAGAAAAAGAAGTTTCTCGTTTAGACCTTGAAGTAGCT
    GGTCATAAAGATGCGGTAAACATGGTAGAGGCAGGCGCTAGTGAGATTACTGAACAAGAA
    ATGTTAGAGGCGATTTTCTTTGGTCATGAAGAGATTCAACGTTTAGTTGATTTCCAACAA
    CAAATCGTCGACCACATTCAACCTGTTAAACAAGAATTTATTCCAGCAGAGCGTGATGAA
    GCGCTAGTTGAACGTGTAAAATCTTTAACCGAAGAAAAAGGACTTAAAGAAACAGTTTTA
    ACATTTGATAAACAACAACGAGATGAAAATCTTGATAACTTAAAAGAAGAAATCGTCAAT
    GAATTTATCGATGAAGAAGATCCAGAGAATGAATTACTTATTAAAGAAGTTTATGCAATT
    TTAAATGAATTAGTGAAAGAAGAAGTTCGACGTTTAATTGCAGATGAAAAAATTAGACCA
    GACGGCCGTAAACCTGATGAAATCCGTCCATTAGATTCTGAAGTTGGTATTTTACCTAGA
    ACGCATGGTTCAGGTCTATTTACACGTGGTCAGACTCAAGCACTTTCAGTTTTAACATTA
    GGTGCTTTAGGCGATTATCAATTAATTGATGGTTTAGGACCTGAAGAAGAAAAAAGATTC
    ATGCATCATTACAACTTCCCGAATTTTTCAGTAGGTGAAACTGGTCCAGTACGTGCGCCA
    GGTCGTCGTGAAATTGGACATGGTGCGTTAGGTGAAAGAGCATTAAAATATATTATTCCT
    GATACTGCTGATTTCCCATATACAATTCGTATTGTAAGTGAGGTACTTGAATCAAATGGT
    TCATCATCTCAAGCGTCAATTTGTGGATCAACATTAGCATTAATGGATGCGGGCGTACCG
    ATTAAAGCACCAGTTGCTGGTATTGCTATGGGCCTTGTTACACGTGAAGATAGCTATACG
    ATTTTAACTGATATCCAAGGTATGGAAGATGCATTAGGTGATATGGACTTTAAAGTCGCT
    GGTACTAAAGAAGGTATTACAGCAATCCAAATGGATATTAAAATTGACGGTTTAACGCGT
    GAAATTATCGAAGAGGCTCTAGAACAAGCGAGACGTGGTCGTTTAGAAATAATGAATCAT
    ATGTTACAAACAATTGATCAACCACGTACTGAATTAAGTGCTTACGCGCCAAAAGTTGTA
    ACTATGACAATTAAACCAGATAAGATTAGAGATGTTATCGGACCTGGTGGTAAAAAAATT
    AACGAAATTATTGATGAAACAGGTGTTAAATTAGATATTGAACAAGATGGTACTATCTTT
    ATTGGTGCTGTTGATCAAGCTATGATAAATCGTGCTCGTGAAATCATTGAGGAAATTACA
    CGTGAAGCGGAAGTAGGTCAAACTTATCAAGCCACTGTTAAACGTATTGAAAAATACGGT
    GCGTTTGTAGGCCTATTCCCAGGTAAAGATGCGTTGCTTCACATTTCACAAATTTCAAAA
    AATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATT
    ACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2097

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01251
  • symbol: SAOUHSC_01251
  • description: polynucleotide phosphorylase/polyadenylase
  • length: 698
  • theoretical pI: 4.60512
  • theoretical MW: 77361.5
  • GRAVY: -0.336103

Function[edit source | edit]

  • reaction:
    EC 2.7.7.8?  ExPASy
    Polyribonucleotide nucleotidyltransferaseRNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate
  • TIGRFAM:
    Genetic information processingTranscriptionDegradation of RNApolyribonucleotide nucleotidyltransferase (TIGR03591; EC 2.7.7.8; HMM-score: 1049)
    guanosine pentaphosphate synthetase I/polyribonucleotide nucleotidyltransferase (TIGR02696; EC 2.7.-.-,2.7.7.8; HMM-score: 683.3)
    Genetic information processingTranscriptionDegradation of RNAexosome complex exonuclease 1 (TIGR02065; EC 3.1.13.-; HMM-score: 121.5)
    Genetic information processingTranscriptionRNA processingribonuclease PH (TIGR01966; EC 2.7.7.56; HMM-score: 57.3)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationribosomal protein bS1 (TIGR00717; HMM-score: 40.5)
    arCOG04150 universal archaeal KH domain protein (TIGR03665; HMM-score: 21.4)
    Genetic information processingTranscriptionDegradation of RNAVacB and RNase II family 3'-5' exoribonucleases (TIGR00358; EC 3.1.13.1; HMM-score: 16.6)
    ATP synthase archaeal, H subunit (TIGR02926; EC 3.6.3.14; HMM-score: 6.1)
  • TheSEED:  
    Bacterial RNA-metabolizing Zn-dependent hydrolases Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) 
  • PFAM:
    S5 (CL0329) RNase_PH; 3' exoribonuclease family, domain 1 (PF01138; HMM-score: 143.7)
    no clan definedRNase_PH_C; 3' exoribonuclease family, domain 2 (PF03725; HMM-score: 90.7)
    OB (CL0021) S1; S1 RNA binding domain (PF00575; HMM-score: 72)
    no clan definedPNPase; Polyribonucleotide nucleotidyltransferase, RNA binding domain (PF03726; HMM-score: 50.2)
    KH (CL0007) KH_1; KH domain (PF00013; HMM-score: 38.7)
    OB (CL0021) S1_2; S1 domain (PF13509; HMM-score: 18)
    TRAM; TRAM domain (PF01938; HMM-score: 14.2)
    no clan definedSPATA25; Spermatogenesis-associated protein 25 (PF15218; HMM-score: 13.7)
    DUF3921; Protein of unknown function (DUF3921) (PF13060; HMM-score: 12.4)
    DUF416; Protein of unknown function (DUF416) (PF04222; HMM-score: 9.9)
    KH (CL0007) KH_2; KH domain (PF07650; HMM-score: 7.4)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.161
    • Ymax_pos: 48
    • Cmax: 0.173
    • Cmax_pos: 48
    • Smax: 0.302
    • Smax_pos: 1
    • Smean: 0.148
    • D: 0.156
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLSTATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPLFPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGKYIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDFQQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKEEIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSEVGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVGETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGSTLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITAIQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKPDKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAEVGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITEIDKQGRVNASHRALEE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 1.57 1.58 1.59 1.60 1.61 1.62 1.63 1.64 1.65 1.66 1.67 1.68 1.69 1.70 1.71 1.72 1.73 1.74 1.75 1.76 1.77 1.78 1.79 1.80 1.81 1.82 1.83 1.84 1.85 1.86 1.87 1.88 1.89 1.90 1.91 1.92 1.93 1.94 1.95 1.96 1.97 1.98 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]