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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS02780 [old locus tag: SACOL0544 ]
  • pan locus tag?: SAUPAN002240000
  • symbol: SACOL_RS02780
  • pan gene symbol?: prs
  • synonym:
  • product: ribose-phosphate pyrophosphokinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL_RS02780 [old locus tag: SACOL0544 ]
  • symbol: SACOL_RS02780
  • product: ribose-phosphate pyrophosphokinase
  • replicon: chromosome
  • strand: +
  • coordinates: 551401..552366
  • length: 966
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002951 (551401..552366) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTTAAATAATGAATATAAGAATTCGTCATTAAAGATTTTTTCATTGAAAGGAAACGAA
    GCATTAGCGCAAGAAGTTGCTGACCAAGTAGGAATTGAACTAGGTAAATGTTCAGTTAAA
    CGTTTTAGTGATGGAGAAATTCAAATTAATATCGAAGAGAGTATTCGTGGTTGTGACGTA
    TTTATTATTCAACCAACATCATATCCTGTGAATCTACATTTAATGGAATTATTAATTATG
    ATTGATGCTTGTAAACGTGCTTCTGCAGCAACAATCAATATTGTAGTGCCATATTATGGA
    TATGCAAGACAAGATAGAAAAGCCCGTAGCCGTAAGCCAATCACTGCTAAATTAGTTGCA
    AACTTAATCGAAACAGCTGGCGCAACTCGTATGATTGCGTTAGACTTACATGCACCACAA
    ATTCAAGGATTCTTTGATATTCCAATTGACCACTTAATGGGTGTGCCAATTCTTGCTAAA
    CATTTCAAAGATGATCCGAATATTAACCCAGAAGAATGTGTCGTTGTTTCACCAGACCAT
    GGCGGCGTTACACGTGCACGTAAATTAGCTGACATTTTAAAAACTCCAATTGCAATTATA
    GATAAACGTCGTCCTAGACCAAATGTTGCTGAAGTGATGAACATTGTTGGTGAGATTGAA
    GGACGTACGGCAATTATTATTGACGATATTATTGATACAGCAGGTACAATCACTTTAGCT
    GCACAAGCATTAAAAGATAAAGGTGCTAAAGAAGTATATGCTTGTTGTACACACCCTGTT
    TTATCAGGACCGGCTAAAGAACGTATCGAAAATTCTGCTATAAAAGAATTAATCGTAACA
    AACTCAATTCATTTAGATGAAGATCGCAAACCATCTAACACTAAAGAATTATCTGTTGCT
    GGTTTAATCGCACAAGCTATCATTCGTGTATACGAAAGAGAATCAGTTAGCGTATTATTT
    GACTAA
    60
    120
    180
    240
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    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    966

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL_RS02780 [old locus tag: SACOL0544 ]
  • symbol: SACOL_RS02780
  • description: ribose-phosphate pyrophosphokinase
  • length: 321
  • theoretical pI: 6.6274
  • theoretical MW: 35282.6
  • GRAVY: -0.0327103

Function[edit source | edit]

  • reaction:
    EC 2.7.6.1?  ExPASy
    Ribose-phosphate diphosphokinaseATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 418.8)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisorotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 26.7)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesSalvage of nucleosides and nucleotideshypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 21.8)
    Cellular processesCellular processesDNA transformationcomF family protein (TIGR00201; HMM-score: 20.5)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisorotate phosphoribosyltransferase (TIGR00336; EC 2.4.2.10; HMM-score: 17.7)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesSalvage of nucleosides and nucleotidesadenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 15.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisamidophosphoribosyltransferase (TIGR01134; EC 2.4.2.14; HMM-score: 13.2)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesSalvage of nucleosides and nucleotidesuracil phosphoribosyltransferase (TIGR01091; EC 2.4.2.9; HMM-score: 12.9)
  • TheSEED:
  • PFAM:
    PRTase-like (CL0533) Pribosyltran_N; N-terminal domain of ribose phosphate pyrophosphokinase (PF13793; HMM-score: 170.5)
    Pribosyl_synth; Phosphoribosyl synthetase-associated domain (PF14572; HMM-score: 98.6)
    Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 75.9)
    UPRTase; Uracil phosphoribosyltransferase (PF14681; HMM-score: 14.8)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.178
    • Ymax_pos: 11
    • Cmax: 0.167
    • Cmax_pos: 24
    • Smax: 0.431
    • Smax_pos: 2
    • Smean: 0.307
    • D: 0.228
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446856519 NCBI
  • RefSeq: WP_000933775 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSDGEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYARQDRKARSRKPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAKHFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGEIEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKELIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SACOL_RS02780 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 1.108 1.109 1.110 1.111 1.112 1.113 1.114 1.115 1.116 1.117 1.118 1.119 1.120 1.121 1.122 1.123 1.124 1.125 1.126 1.127 1.128 1.129 1.130 1.131 1.132 1.133 1.134 1.135 1.136 1.137 1.138 1.139 1.140 1.141 1.142 1.143 1.144 1.145 1.146 1.147 1.148 1.149 1.150 1.151 1.152 1.153 1.154 1.155 1.156 1.157 1.158

Relevant publications[edit source | edit]