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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06795 [old locus tag: NWMN_1208 ]
  • pan locus tag?: SAUPAN003612000
  • symbol: NWMN_RS06795
  • pan gene symbol?: glpK
  • synonym:
  • product: glycerol kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS06795 [old locus tag: NWMN_1208 ]
  • symbol: NWMN_RS06795
  • product: glycerol kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1333237..1334733
  • length: 1497
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1333237..1334733) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
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    361
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    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAAAAATATATTTTATCTATAGACCAAGGAACAACAAGCTCAAGAGCGATTTTATTC
    AATCAAAAAGGGGAAATTGCAGGGGTAGCACAACGTGAGTTTAAGCAATATTTTCCACAA
    TCAGGTTGGGTTGAACATGATGCAAATGAAATTTGGACATCTGTGTTAGCTGTAATGACG
    GAAGTAATTAATGAAAATGATGTTAGAGCTGATCAAATTGCAGGTATCGGTATTACAAAC
    CAACGTGAAACAACGGTTGTTTGGGACAAACATACTGGCCGCCCAATTTATCACGCAATT
    GTTTGGCAATCACGTCAAACACAATCAATTTGTTCAGAATTAAAACAACAAGGATATGAA
    CAAACATTTAGAGATAAGACAGGATTACTTTTAGATCCGTATTTTGCAGGTACAAAAGTT
    AAATGGATTCTAGACAATGTTGAAGGTGCACGAGAAAAAGCAGAAAATGGCGATCTATTA
    TTTGGAACGATTGATACTTGGTTAGTATGGAAATTATCAGGAAAAGCTGCGCATATTACT
    GATTATTCAAATGCGAGTCGTACATTAATGTTTAATATCCATGATTTAGAATGGGACGAT
    GAGTTATTAGAACTACTTACAGTACCTAAAAATATGTTGCCAGAAGTTAAAGCTTCGAGT
    GAAGTATATGGTAAGACAATTGATTACCACTTCTATGGTCAAGAAGTACCAATCGCTGGA
    GTAGCTGGTGATCAACAAGCAGCATTATTTGGACAAGCTTGCTTCGAACGTGGTGACGTG
    AAAAACACATATGGAACTGGTGGCTTCATGTTAATGAATACAGGTGACAAAGCGGTTAAA
    TCTGAAAGTGGTTTATTAACAACAATTGCTTATGGTATTGATGGAAAAGTAAATTATGCG
    CTTGAAGGTTCCATCTTTGTTTCGGGTTCAGCAATCCAATGGTTACGTGATGGATTAAGA
    ATGATTAATTCAGCACCACAATCAGAAAGTTATGCGACACGAGTTGACTCTACTGAGGGT
    GTTTATGTTGTTCCAGCTTTTGTAGGTTTAGGAACACCATATTGGGATTCTGAAGCACGT
    GGTGCGATTTTCGGTTTAACACGTGGAACTGAAAAAGAGCACTTTATCCGTGCAACTTTA
    GAATCACTATGTTACCAAACTCGTGACGTTATGGAAGCAATGTCAAAAGACTCTGGTATT
    GATGTCCAAAGTTTACGTGTCGATGGTGGTGCAGTTAAAAATAACTTTATTATGCAGTTC
    CAAGCAGACATTGTTAATACTTCTGTTGAAAGACCTGAAATTCAAGAAACTACAGCTTTA
    GGTGCTGCATTTTTAGCAGGTTTAGCAGTTGGATTCTGGGAGAGTAAAGATGATATCGCT
    AAAAACTGGAAATTAGAAGAAAAATTCGATCCGAAAATGGATGAAGGCGAAAGAGAAAAA
    TTATATAGAGGTTGGAAAAAAGCTGTTGAAGCAACACAAGTTTTTAAAACAGAATAA
    60
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    1440
    1497

Protein[edit source | edit]

Protein Data Bank: 3G25
Protein Data Bank: 3GE1

General[edit source | edit]

  • locus tag: NWMN_RS06795 [old locus tag: NWMN_1208 ]
  • symbol: NWMN_RS06795
  • description: glycerol kinase
  • length: 498
  • theoretical pI: 4.6683
  • theoretical MW: 55625.2
  • GRAVY: -0.340964

Function[edit source | edit]

  • reaction:
    EC 2.7.1.30?  ExPASy
    Glycerol kinaseATP + glycerol = ADP + sn-glycerol 3-phosphate
  • TIGRFAM:
    MetabolismEnergy metabolismOtherglycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 760.2)
    MetabolismEnergy metabolismSugarsxylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 240.7)
    MetabolismEnergy metabolismSugarsgluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 173.5)
    MetabolismEnergy metabolismSugarsL-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 120.9)
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 109.4)
    MetabolismEnergy metabolismSugarsribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 68.5)
    rhamnulokinase (TIGR02627; EC 2.7.1.5; HMM-score: 57.7)
    TraF-like protein (TIGR02740; HMM-score: 12.8)
  • TheSEED:
  • PFAM:
    Actin_ATPase (CL0108) FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 296.5)
    FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 215.8)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 19.1)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.137
    • Ymax_pos: 33
    • Cmax: 0.132
    • Cmax_pos: 33
    • Smax: 0.195
    • Smax_pos: 26
    • Smean: 0.116
    • D: 0.129
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446339514 NCBI
  • RefSeq: WP_000417369 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLLFGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKASSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS06795 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 1.108 1.109 1.110 1.111 1.112 1.113 1.114 1.115 1.116 1.117 1.118 1.119 1.120 1.121 1.122 1.123 1.124 1.125 1.126 1.127 1.128 1.129 1.130 1.131 1.132 1.133 1.134 1.135 1.136 1.137 1.138 1.139 1.140 1.141 1.142 1.143 1.144 1.145 1.146 1.147 1.148 1.149 1.150 1.151 1.152 1.153 1.154 1.155 1.156 1.157 1.158 1.159 1.160 1.161 1.162 1.163 1.164 1.165 1.166 1.167 1.168 1.169 1.170 1.171 1.172 1.173 1.174 1.175 1.176 1.177 1.178 1.179 1.180 1.181 1.182 1.183 1.184 1.185 1.186 1.187 1.188 1.189 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

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